METHOD FOR THE SEPARATION AND PURIFICATION OF NUCLEIC ACID

Japanese Patent JP3819001

B2

PROBLEM TO BE SOLVED: To provide an analytical method for nucleic acid fragments capable of simply and rapidly carrying out free from needs of any specific technique, complicated operation and specific device, by using a small device, namely, an analytical method for nucleic acid fragments which is capable of space-saving and automating.
SOLUTION: The analytical method comprises following steps. Step (1), a step isolating and purifying nucleic acid fragment containing the target nucleic acid fragment including a step making a nucleic acid adsorbed onto a solid phase composed of an organic high polymer having a hydroxy group on a surface thereof, and desorbed from the solid phase. (2), a step allowing the target nucleic acid fragment, at least one primer complementary to a portion of the target nucleic acid fragment, at least one deoxynucleoside triphosphate, and at least one polymerase to react with each other, to conduct polymerase elongation reaction with using the target nucleic acid fragment as a template and using 3' terminal of the primer as initiation site. And (3) a step detecting whether polymerase elongation reaction proceeds or whether the polymerase elongation reaction product hybridizes with another nucleic acid.

Toshihiro Mori
Yumiko Takeshita
Yoshihiko Makino

JP2004000345A

September 06, 2006

January 05, 2004

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Fuji Photo Film Co., Ltd.

C12N15/09; G01N33/50; C12M1/00; C12M1/34; C12Q1/26; C12Q1/42; C12Q1/48; C12Q1/68; G01N33/52; G01N33/53; G01N33/566; (IPC1-7): C12Q1/68; C12M1/00; C12M1/34; C12N15/09; C12Q1/26; C12Q1/42; C12Q1/48; G01N33/50; G01N33/52; G01N33/53; G01N33/566

JP2000325082A

Anal Biochem 254 p.18-22 (1997)
Biochem J 229 p.255-260 (1985)

Shohei Oguri
Hironori Honda
Toshimitsu Ichikawa
Takeshi Takamatsu

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