Title:
非天然ヌクレオチドの取り込みによるインビトロの核酸の部位特異的な酵素標識のための方法
Document Type and Number:
Japanese Patent JP7383674
Kind Code:
B2
Abstract:
Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs that form unnatural base pairs during DNA polymerase-mediated replication of DNA or RNA polymerase-mediated transcription of RNA. In this manner, the unnatural nucleobases can be introduced in a site-specific way into oligonucleotides (single or double stranded DNA or RNA), where they can provide for site-specific cleavage, or can provide a reactive linker than can undergo functionalization with a cargo-bearing reagent by means of reaction with a primary amino group or by means of click chemistry with an alkyne group of the unnatural nucleobase linker.
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Inventors:
Romsburg, Floyd, E.
Marui Chef, Dennis, A.
Lee, lin jun
Raven, thomas
Lee, Centao
Marui Chef, Dennis, A.
Lee, lin jun
Raven, thomas
Lee, Centao
Application Number:
JP2021147954A
Publication Date:
November 20, 2023
Filing Date:
September 10, 2021
Export Citation:
Assignee:
THE SCRIPPS RESEARCH INSTITUTE
International Classes:
C07H21/04; C12N15/11; C12N15/113
Domestic Patent References:
| JP2019196355A |
Other References:
J. Am. Chem. Soc.,2009年,131,5046-5047
Attorney, Agent or Firm:
Kiyohara International Patent Office
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