METHOD AND SYSTEM FOR USE IN DIRECT SEQUENCING OF RNA

Title:

METHOD AND SYSTEM FOR USE IN DIRECT SEQUENCING OF RNA

Document Type and Number:

Japanese Patent JP2023156389

Kind Code:

A

Abstract:

To provide a system and a method for determining the order of nucleotides of an RNA molecule.SOLUTION: A method comprises the steps of: receiving liquid chromatography-mass spectrometry (LC-MS) data of an RNA sample; filtering the LC-MS data on the basis of mass, the filtering including removing masses smaller than a predetermined size; analyzing the filtered LC-MS data to determine a plurality of RNA sequences; and reading out an RNA sequence after determining no remaining valid nucleotide in the remaining LC-MS data. The step of analyzing the filtered LC-MS data includes determining a mass difference between at least two adjacent ladder fragments and determining whether the mass difference is equal to a canonical nucleotide or a modified nucleotide.SELECTED DRAWING: None

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Inventors:

ZHANG SHENGLONG
TOM Z WANG
TONY Z JIA
LI WENJIA

Application Number:

JP2023126160A

Publication Date:

October 24, 2023

Filing Date:

August 02, 2023

Export Citation:

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Assignee:

NEW YORK INSTITUTE OF TECH

International Classes:

G01N27/62; C12N15/11; C12Q1/6872; G16B30/00

Attorney, Agent or Firm:

Shusaku Yamamoto
Natsuki Morishita
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto

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