Title:
METHOD FOR ULTRAFILTERING BIOLOGICAL SUBSTRATE CONTAINING PEPTIDE OR PROTEIN
Document Type and Number:
Japanese Patent JP3218193
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To provide the method which is appropriately used for desalting and concentrating a culture broth to required levels for the next purification process respectively with a membrane having high peptide or protein retentivity, in order to perform the prepurification of a fermentation broth contg. peptides or proteins, in particular, desalting and concentration of the broth.
SOLUTION: In this method, a broth is subjected to ultrafiltration with a membrane having a specified separation limit (molecular-weight cutoff value) within the range of 2 to 5 of the molecular weight of peptides or proteins to be retained on the membrane to subject the culture broth contg. peptides or proteins and not contg. bacteria to prepurification.
Inventors:
Jerck Mellar
Frank Richard
Frank Richard
Application Number:
JP31154296A
Publication Date:
October 15, 2001
Filing Date:
November 22, 1996
Export Citation:
Assignee:
Aventis Pharma Deutsche Land Gesellschaft Mitt Beschlenktel Haftung
International Classes:
B01D61/14; C07K1/14; C07K1/34; C07K14/815; C12P21/04; (IPC1-7): C07K1/34; C07K14/815
Domestic Patent References:
| JP6145198A | ||||
| JP748399A | ||||
| JP6222799A | ||||
| JP60233098A |
Attorney, Agent or Firm:
Chika Takagi (1 person outside)