Title:
MICROFLUIDIC NUCLEIC ACID ANALYSIS
Document Type and Number:
Japanese Patent JP2014168480
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide microfluidic nucleic acid analysis.SOLUTION: Nucleic acid from cells and viruses sampled from a variety of environments may be purified and expressed utilizing microfluidic techniques. In accordance with one embodiment of the present invention, individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. In one specific application, cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.
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Inventors:
HONG JONG WOOK
STUDER VINCENT
ANDERSON W FRENCH
QUAKE STEPHEN R
LEADBETTER JARED
STUDER VINCENT
ANDERSON W FRENCH
QUAKE STEPHEN R
LEADBETTER JARED
Application Number:
JP2014121156A
Publication Date:
September 18, 2014
Filing Date:
June 12, 2014
Export Citation:
Assignee:
CALIFORNIA INST OF TECHN
International Classes:
C12M1/00; B01L3/00; C12M1/34; C12N15/09; C12Q1/68
Attorney, Agent or Firm:
Shusaku Yamamoto
Natsuki Morishita
Natsuki Morishita