PURPOSE: The titled cellular surface antigen recognition type antibody, produced by a hybridoma prepared by using a cellular strain (MKN-45) derived from a gastric low differentiation type gladular cancer as an immunization source, having a light chain of IgG1(K) and capable of exhibiting positive reaction to cancer cells, e.g. specific gastric cancer or esophageal cancer.
CONSTITUTION: A monoclonal antibody obtained by fractionating a cell extract of an established cell (MKN-45) derived from a gastric low differentiation type glandular cancer, mixing the resultant high-molecular fraction having 700,000W1,500,000mol.wt. with complete Freund's adjuvant, immunizing an animal, e.g. mouse, with the resultant mixture, collecting a cell producing an antibody, carrying out cell fusion with a myelomatous cell of an animal of the same species, cloning the resultant cell to give a cloned hybridoma, transplanting the resultant cloned hybridoma to the abdominal cavity of an animal, e.g. BALB/C mouse, to produce the aimed antibody capable of exhibiting positive reaction to cancer cells of gastric cancer (MKN-45 or KATO-III), esophageal cancer (TE-3), lung cancer (PC-10) and colonic cancer (Colo201) and recovering and purifying the antibody from the ascites.
KAMIMURA YATSUHIRO
FUKUYAMA KAZUMI
KOBAYASHI TAKASHI
KANO YOSHIAKI
YAMANA RYUTARO
TANIGUCHI TOMOKUNI
NAGURA KAZUAKI
WATANABE MASAHIRO
NISHIDA MASAYUKI
SUYAMA TADAKAZU
| JPS61167699A | 1986-07-29 |