Title:
MUTATED IMMUNOGLOBULIN-BINDING POLYPEPTIDES
Document Type and Number:
Japanese Patent JP2020184999
Kind Code:
A
Abstract:
To provide mutated immunoglobulin-binding domains of Protein A useful in the field of affinity chromatography, more particularly, useful in the affinity chromatography of immunoglobulins, as well as multimers of mutated domains and separation matrix comprising the mutated domains or multimers thereof.SOLUTION: Disclosed is an Fc binding polypeptide having improved alkali stability comprising a mutant of Fc binding domain of Staphylococcal Protein A (SpA), as defined by one of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO 26 or SEQ ID NO 27, where at least the alanine residue at position 42 in SEQ ID NOs: 4-7 has been mutated to arginine and/or at least the asparagine residue at position 37 of SEQ ID NOs: 4-7 has been mutated to glutamic acid.SELECTED DRAWING: Figure 1
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Inventors:
GUSTAV RODRIGO
TOMAS BJORKMAN
MATS ANDER
TOMAS BJORKMAN
MATS ANDER
Application Number:
JP2020121312A
Publication Date:
November 19, 2020
Filing Date:
July 15, 2020
Export Citation:
Assignee:
CYTIVA BIOPROCESS R & D AB
International Classes:
C12N15/31; B01D15/38; B01J20/281; B01J20/285; C07K1/22; C07K14/31; C07K16/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/63; G01N30/50; G01N30/88
Domestic Patent References:
| JP6770727B2 | 2020-10-21 | |||
| JP2005538693A | 2005-12-22 | |||
| JPH02500329A | 1990-02-08 |
Foreign References:
| WO2014046278A1 | 2014-03-27 | |||
| WO2011118699A1 | 2011-09-29 | |||
| WO2012133349A1 | 2012-10-04 | |||
| US20060194955A1 | 2006-08-31 | |||
| EP2202310A2 | 2010-06-30 |
Other References:
TOVE BOSTROM: "PURIFICATION SYSTEMS BASED ON BACTERIAL SURFACE PROTEINS", PROTEIN PURIFICATION, JPN5017009410, 20 January 2012 (2012-01-20), pages 89 - 136, ISSN: 0004567459
Attorney, Agent or Firm:
Masato Iida
Choi
Kenji Tanaka
Choi
Kenji Tanaka