PURPOSE: To obtain a new compound which is a nucleotide, corresponding to four bases of adenine, cytosine, guanine and thymine useful for determining the base sequence of nucleic acid and capable of readily preparing an intermediate for a nucleoside before marking thereof with a labeling substance.
CONSTITUTION: This compound of formula I (R1 to R4 are each H, Na or Li, with the proviso that Na and Li are not simultaneously present; A is formulas II, III, etc.), e.g. 7-(N-biotinyl-3-amino-1-propynyl)-2',3'-dideoxy-7-deazaadenosine 5'-triphosphate. The compound is obtained by iodinating the 5-position in a pyrimidine base of a nucleoside or the 7-position in a deazapurine base, then reacting the resultant compound with N-trifluoroacetylpropargylamine in the presence of a Pd 0-valent catalyst, providing a nucleoside of formula IV (Q is a cytosine or a uracil residue, etc.), triphosphating the OH at the 5'-terminal thereof and then reacting the obtained precursor with a biotinyl-N-succinimide ester.
IIJIMA AYAKO
MIZUKOSHI TAKAYOSHI
Next Patent: RUTHENIUM CARBONYL METALLIC CLUSTER COMPLEX