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Title:
NEW CDNA-CLONING VECTOR
Document Type and Number:
Japanese Patent JPH02186991
Kind Code:
A
Abstract:

PURPOSE: To obtain recombinant DNA molecules useful for the high frequency cloning of double stranded cDNA and the synthesis of single stranded cDNA and capable of replicating in procaryote-hosts and, if needed, eucaryote hosts.

CONSTITUTION: This new cDNA cloning vector is a recombinant DNA molecule consisting of the following components; (A) a replicator of procaryote, (B) a selectable marker, (C) a deoxyribonucleic acid arrangement (D) the only one restrictive endonuclease part capable of (i) recyclizing through an intramolecular binding of cDNA-containing recombinant DNA molecule and (ii) starting a cDNA-synthesis of the second chain.


Inventors:
SUTEIIBUN SHII PURUTSUTO
Application Number:
JP15353489A
Publication Date:
July 23, 1990
Filing Date:
June 14, 1989
Export Citation:
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Assignee:
HERUSU RES INC
International Classes:
C12N1/21; C12N15/10; C12N15/64; C12N15/70; C12R1/19; (IPC1-7): C12N1/21; C12N15/63; C12N15/66
Attorney, Agent or Firm:
Aoyama Ryo (1 person outside)