NEW MATERIAL:A cDNA clone such as phage clone YS1 or phage clone YS2 containing a nucleotide of about 5.4Kb coding the amino acid sequence of a post-transfusion non-A non-B hepatitis virus (NANB) and prepared by adding an EcoRI linker to a duplex cDNA fragment prepared by using a template consisting of a refined RNA existing in a particle fraction separated from serum and substituting and inserting the addition product to an EcoRI site of a lambda gt10 vector.
USE: Agent for the diagnosis, prevention and remedy of non-A non-B hepatitis. Blood cleaning agent for transfusion.
PREPARATION: The objective cDNA clone can be prepared e.g. by adding 20% polyethylene glycol to a plasma originated from a non-A non-B hepatitis patient, centrifuging the mixture at a high speed, solubilizing the precipitate with TEN buffer solution, etc., centrifuging at a high speed to collect purified RNA, preparing a duplex cDNA using the RNA as a template, adding an EcoRI linker to the cDNA and substituting and inserting the addition product to the EcoRI site of a lambda gt10 vector.
MURAKAMI SEISHI
INAGAKI YUTAKA
YANAGI MASAYUKI
SAWAI KIICHI
