Title:
New cellulase gene
Document Type and Number:
Japanese Patent JP6106738
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To identify an endoglucanase gene or a β-glucosidase gene by: isolating genomic DNA containing a cellulase gene that is classified as the endoglucanase or the β-glucosidase, from Acremonium cellulolyticus; and analyzing its base sequence.SOLUTION: Amino acid sequences of known endoglucanases and β-glucosidases are compared with each other assiduously, and an amino acid sequence which can be conserved in Acremonium cellulolyticus is found. Based on the information, various primers are designed. Using the various primers thus designed, a PCR is carried out employing genomic DNA or cDNA as a template. As a result, fragments of genes for endoglucanases and genes for β-glucosidases can be obtained. Thus primers are designed based on the gene fragments, the PCR is continued to amplify nine endoglucanase and β-glucosidase genes, and base sequences are analyzed to achieve the present invention.SELECTED DRAWING: None
Inventors:
Yokoyama Fumikazu
Application Number:
JP2015227865A
Publication Date:
April 05, 2017
Filing Date:
November 20, 2015
Export Citation:
Assignee:
meiji seika Pharma Co., Ltd.
International Classes:
C12N9/42; A23K20/00; C12N1/13; C12N1/15; C12N1/19; C12N5/10; C12N15/09; C12P19/14; D21C9/00
Domestic Patent References:
| JP2000069978A | ||||
| JP10066569A |
Foreign References:
| WO1997033982A1 | ||||
| WO1999011767A1 |
Other References:
B6QKD0,UniProtKB[online],2008年12月16日,[検索日 2016年8月10日],URL,http://www.uniprot.org/uniprot/B6QKD0
SCHULEIN, M. et al.,Book of Abstracts, 213th ACS National Meeting, San Francisco, April 13-17,1997年,[abstract] Chemical Abstracts [online], Accession No.1997:159095
SCHULEIN, M. et al.,Book of Abstracts, 213th ACS National Meeting, San Francisco, April 13-17,1997年,[abstract] Chemical Abstracts [online], Accession No.1997:159095
Attorney, Agent or Firm:
Kenichi Morita
Kenjiro Yamaguchi
Kenjiro Yamaguchi
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