NEW CHITINASE AND ITS PRODUCTION - Marine Biotechnology Research Institute Co., Ltd.

Title:

NEW CHITINASE AND ITS PRODUCTION

Document Type and Number:

Japanese Patent JP3820418

Kind Code:

B2

Abstract:

PURPOSE: To obtain a new chitinase, capable of decomposing chitin and important for effective utilization of the chitin.
CONSTITUTION: This chitinase C-l or C-3 is obtained by culturing a Vibrio sp. H-8 strain (FERM P-14737), etc., and having the following properties: (l) each capable of acting on chitin and hydrolyzing the chitin into a soluble N- acetylchitooligosaccharide; (2) optimal pH7.8-8.2 (40°C), 50°C optimal temperature (pH8.0), stable by treatment at pH8.0 and 50°C for 1hr, about 69000 molecular weight [measured according to a sodium dodecyl sulfate-polyacrylamide gel electrophoretic method (SDS-PAGE)] and 3.6 isoelectric point for the chitinase C-l; (3) optimal pH7.8-8.2 (40°C), 50°C optimal temperature (pH8.0), stable by treatment at pH8.0 and 50°C for 1hr, about 81000 molecular weight (measured according to the SDS-PAGE method) and 3.9 isoelectric point for the chitinase C-3, etc., and (4) each having high activities.

Inventors:

Hitoshi Izumida
Hiroshi Sano
Miyuki Nishijima
Kazuo Oishi
Masaaki Yamagishi
Mitsuaki Suzuki
Atsuhiro Katsuyama
Miwa end

Application Number:

JP8107895A

Publication Date:

September 13, 2006

Filing Date:

April 06, 1995

Export Citation:

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Assignee:

Marine Biotechnology Research Institute Co., Ltd.
Shizuoka Prefecture
KAI Kasei Co., Ltd.

International Classes:

C12N9/24; C12N9/42; C12R1/63; (IPC1-7): C12N9/24; //(C12N9/24; C12R1:63)

Domestic Patent References:

JP6113846A

Other References:

J. Ferment. Technol.,1979年,57 [3],p.169-177
Biosci. Biotech. Biochem.,1992年,56 [2],p.368-369

Attorney, Agent or Firm:

Yusuke Hiraki
Ishii Teiji

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