To provide a method of detecting the concentration of glucose noninvasively through an analyte, for example, a human skin using a hyperosmotic solution in a method of determining the concentration of the analyte.

A non-invasive method of measuring the concentration of an analyte, comprises topographically applying a hyperosmotic solution to an area of the skin. The hyperosmotic solution is adapted to at least partially absorb into the area of the skin such that the skin becomes generally transparent. An optical readhead is placed over the generally transparent area of the skin. The amount of light of the analyte is measured using at least one wavelength via the optical readhead. The concentration of the analyte is calculated from the amount of light. The analyte may be glucose and the hyperosmotic solution may be glycerol.