To provide a method for detecting the presence or amount of an analyte in a sample.
The method includes: a porous surface to which particles having attached thereto a binding substance, analyte and binding substance coated label particles are added. If the analyte is present in the sample, an immuno- or chemical reaction occurs in the liquid phase. The separation of bound complex from unbound material is achieved by using the surface where the separation occurs mainly two dimensionally on the surface of the porous surface (e.g., grid). The disclosed surface enables a separation where the complexes are distributed two dimensionally on the porous surface (e.g., grid), whereas unbound materials are distributed three dimensionally. Accordingly, the surface enables both a two and a three dimensional separation.
NIKULA HANNU
MURTOVUORI MIRA
Koichi Niimi
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