To obtain an agent containing a water soluble polysaccharide produced by a filamentous fungus belonging to the genus Pestalotiopsis or Pestalotia and used as a blood sugar reducing agent, a suppressing agent for blood sugar elevation and a blood cholesterol reducing agent.
This physiologically active agent contains a water soluble polysaccharide contained in a cultured medium obtained by culturing a filamentous fungus belonging to the genus Pestalotiopsis or Pestalotia, e.g. Pestalotiopsis sp KGM96004. The water soluble polysacchairde is blended preferably as a form of a crude material or a purified material. In order to prepare the purified material, a four step process are performed by mixing the cultured medium of the filamentous fungus with a polar organic solvent to obtain a precipitated material, mixing the precipitated material with water, performing a centrifugal separation to obtain the supernatant, adsorbing the supernatant with an anion exchanger, collecting an eluting fraction by flowing a buffer solution therethrough, forming a dried material by concentrating and drying the fraction, dissolving the dried material with water, performing the centrifugal separation to separate and collect the supernatant, and concentrating.
KIKATA TADASHI
OKAMOTO TAKAMITSU
MORI YOSHINOBU
SAKAMOTO HIDEKI
