Title:
PREPARATION AND USE OF LIPOOLIGOSACCHARIDE-DEPLETED OUTER MEMBRANE PROTEIN OF GRAM-NEGATIVE COCCUS
Document Type and Number:
Japanese Patent JP3711289
Kind Code:
B2
Abstract:
PURPOSE: To provide a method for preparing the subject outer membrane protein usable as bacteriocidal antibody inducer by which a lipooligosaccharide is depleted by extracting the outer membrane without the inner membrane from the whole membrane of a Gram-negative cocci with a specific nonionic surfactant, and extraction-treating the outer membrane with an amphoteric surfactant.
CONSTITUTION: Gram-negative cocci (e.g. Neisseria menigitidis) are freeze-dried to be extinct, suspended in a hypotonic solution, and subjected to a centrifugal separation to separate a cytoplasm extract as a supernatant and to gather the whole membrane as a pellet. The whole membrane of the cocci is subjected to an extraction treatment with a 1% solution of a polyoxyethylene surfactant to remove the inner membrane and to prepare the outer membrane of the cocci. The obtained outer membrane is subjected to the extraction treatment with a buffer solution containing 1% amphoteric ionic betain surfactant of the formula [R1 is a 10-16C alkyl: R2 is carboxyl or sulfonyl; (n) is ≥1, preferably 2 or 3] to remove lipooligosaccharide and to provide the objective outer membrane protein effective for treatment or the like of bacterial meningitis or the like and capable of inducing bacteriocidal antibody.
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Inventors:
Gary D'Abrieu Zlotnik
Application Number:
JP12203294A
Publication Date:
November 02, 2005
Filing Date:
May 12, 1994
Export Citation:
Assignee:
Wyeth Holdings Corporation
International Classes:
C12P21/00; A61K39/09; A61K39/095; A61K39/395; A61P31/04; C07K14/195; C07K14/22; C07K14/41; C07K16/14; A61K39/00; C12R1/36; (IPC1-7): C12P21/00; A61K39/095; A61P31/04; C07K14/22; C07K16/14
Foreign References:
| WO1990010458A1 |
Attorney, Agent or Firm:
Heikichi Odashima