Title:
PROCESS AND REAGENT FOR OLIGONUCLEOTIDE SYNTHESIS AND PURIFICATION
Document Type and Number:
Japanese Patent JP2008208135
Kind Code:
A
Abstract:
To provide an improved synthesis process, a process usable for purification and a reaction reagent, for efficiently performing oligonucleotide synthesis.
Compounds that scavenge acrylonitrile produced during the deprotection of phosphate groups bearing ethylnitrile protecting groups, are provided. In a preferred embodiment, the acrylonitrile scavenger is a polymer-bound thiol. In another aspect, the reaction agent used to oxidize a phosphite to a phosphate is provided. In a preferred embodiment, the oxidizing agent is sodium chlorite, chloroamine or pyridine-N-oxide.
Inventors:
MANOHARAN MUTHIAH
JUNG MICHAEL E
RAJEEV KALLANTHOTTATHIL G
PANDEY RAJENDRA
WANG GANG
JUNG MICHAEL E
RAJEEV KALLANTHOTTATHIL G
PANDEY RAJENDRA
WANG GANG
Application Number:
JP2008104338A
Publication Date:
September 11, 2008
Filing Date:
April 14, 2008
Export Citation:
Assignee:
ALNYLAM PHARMACEUTICALS INC
International Classes:
C07H21/02; C07D285/01; C07H21/00; C07H21/04; C07D285/00; C12N15/09
Domestic Patent References:
| JP2000226399A | 2000-08-15 | |||
| JP2004503561A | 2004-02-05 |
Foreign References:
| WO2002072864A2 | 2002-09-19 |
Attorney, Agent or Firm:
Yanagita Seiji
Go Sakuma
Go Sakuma