PURPOSE: To obtain the title compound useful as an immunosuppressive agent efficiently and stably by culturing an undifferentiated cell derived from a plant belonging to the genus Leucosceptrum and separating and collecting acteoside from the cultured cell.
CONSTITUTION: A leaf or genital organ of a plant belonging to the genus Leucosceptrum of the family Labiatae or part thereof is sterilized by a method of using an aqueous solution of ethanol, cut into a proper size and cultured in a liquid medium or solid medium mainly comprising an inorganic synthetic medium blended with a plant growth regulator such as saccharide, e.g. sucrose or indoleacetic acid and vitamins, etc., at 15-30°C for 2-8 weeks to give a callus. The callus is subjected to subculture to give a large amount of undifferentiated cell. The cell is extracted, isolated and purified to give the aimed compound.
NISHIMURA HIROAKI
OKADA MINORU
MIHASHI HIROSHI
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