Title:
PRODUCTION OF NEOTREHALOSE AND ITS USE
Document Type and Number:
Japanese Patent JP3172925
Kind Code:
B2
Abstract:
PURPOSE: To obtain in an industrially advantageous way neotrehalose, a non- reducing disaccharide having good-quality, profound sweetness, serving as an energy source, thus useful as a sweetener by making α-amylase act on a starch substance.
CONSTITUTION: A starch substance (e.g. dextrin) is dissolved in hot water and the pH level of the resulting solution is adjusted to 5.6; 100 units per g of the starch substance, of a saccharifying α-amylase is added to the solution at 60°C followed by reaction for 20hr and then heating to deactivate the enzyme. The resulting reaction liquor containing neotrehalose formed is conventionally decolored using active carbon. Then, the resultant liquor is put to a column chromatography using a salttype strongly acidic cation exchange resin to collect a fraction of neotrehalose content, which is, in turn, concentrated to crystallize and collect the neotrehalose, thus industrially obtaining the objective neotrehalose highly utilizable metabolically and having the above-mentioned advantages.
Inventors:
Takashi Shibuya
Hiroto Chaen
Shuzo Sakai
Toshio Miyake
Hiroto Chaen
Shuzo Sakai
Toshio Miyake
Application Number:
JP9393692A
Publication Date:
June 04, 2001
Filing Date:
February 25, 1992
Export Citation:
Assignee:
Hayashibara Institute of Biochemistry
International Classes:
A23C9/152; A23C9/13; A23G1/00; A23G1/30; A23G3/00; A23G3/34; A23G4/00; A23L2/39; A23L9/20; A23L15/00; A23L21/10; A23L27/30; A23L33/20; A61K9/28; A61K31/70; A61P3/00; A61P7/00; A61P17/00; C12P19/12; C12P19/14; (IPC1-7): C12P19/14
Domestic Patent References:
| JP63216492A |