Title:
PURIFIED NEW GOAT EPIDIDYMAL ADVANCING PROTEIN AND PURIFICATION OF THE PROTEIN USEFUL AS FERTILITY PROMOTING FACTOR/SUPPRESSING FACTOR
Document Type and Number:
Japanese Patent JP3400367
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To obtain the subject new protein isolated from goat epididymal plasma, comprising an advancing protein having specific molecular weight, having fertility promoting/suppressing action, useful for treating human infertility, breeding of domestic animal, etc.
SOLUTION: This new forward motility protein is isolated from goat epididymal plasma, has 125 KDA molecular weight, useful as a fertility promoting/suppressing factor and is used for treating human infertility and solving problems with respect to breeding of domestic animal, etc., by improving a sperm by the improved advancing motility. The protein is obtained by collecting a goat epididymal tail part, separating plasma from the epididymal tail part, fractionating the plasma with ammonium sulfate, collecting a precipitate at 30-70% saturation of the salt, subjecting the plasma to an anion exchange resin by using a low ion alkali buffer, eluting an adsorbed substance and further purifying the eluted fraction by concanavalin affinity chromatography.
Inventors:
Gopal Chandra Majumdel
Vijay Shankar Jaiswal
Vijay Shankar Jaiswal
Application Number:
JP36625798A
Publication Date:
April 28, 2003
Filing Date:
December 24, 1998
Export Citation:
Assignee:
Council of Scientific and Industrial Research
International Classes:
A61K38/00; A61K31/00; A61K38/14; A61P15/00; A61P15/16; A61P37/04; C07K1/16; C07K1/18; C07K1/22; C07K14/47; (IPC1-7): C07K14/47; C07K1/16; C07K1/18; C07K1/22
Other References:
Ｂｉｏｌ．Ｒｅｐｒｏｄ．，Ｖｏｌ．１９（４），ｐ．８３０−８３５（１９７８）
Ｂｉｏｌ．Ｒｅｐｒｏｄ．，Ｖｏｌ．２０（２），ｐ．２４７−２５２（１９７９）
Ｉｎｔ．Ｊ．Ａｎｄｒｏｌ．，Ｖｏｌ．９（５），ｐ．３７１−３８０（１９８６）
Ｊ．Ｂｉｏｌ．Ｃｈｅｍ．，Ｖｏｌ．２５３（１９），ｐ．６７４４−６７５０（１９７８）
Ｂｉｏｌ．Ｒｅｐｒｏｄ．，Ｖｏｌ．２０（２），ｐ．２４７−２５２（１９７９）
Ｉｎｔ．Ｊ．Ａｎｄｒｏｌ．，Ｖｏｌ．９（５），ｐ．３７１−３８０（１９８６）
Ｊ．Ｂｉｏｌ．Ｃｈｅｍ．，Ｖｏｌ．２５３（１９），ｐ．６７４４−６７５０（１９７８）
Attorney, Agent or Firm:
Minoru Nakamura (6 outside)