Title:
Refining of non-glycosylating protein
Document Type and Number:
Japanese Patent JP5944101
Kind Code:
B2
Abstract:
The current invention reports a method for the purification of a not-glycosylated, heterologous polypeptide, which has been recombinantly produced in a prokaryotic cell, wherein the method comprises three chromatography steps of which the first chromatography step selected from i) hydrophobic charge induction chromatography, or ii) hydrophobic interaction chromatography, or iii) affinity chromatography, or iv) ion exchange chromatography, the second chromatography step is selected from i) anion exchange chromatography, or ii) cation exchange chromatography, or iii) hydroxylapatite chromatography, or iv) hydrophobic interaction chromatography, and the a third chromatography step is selected from i) hydrophobic charge induction chromatography, or ii) anion exchange chromatography, or iii) cation exchange chromatography, or iv) hydrophobic interaction chromatography, whereby the first chromatography step is an affinity chromatography in case of polypeptides capable of interacting with metal ligands, the second chromatography step is not a hydroxylapatite chromatography step in case of polypeptides with an isoelectric point below 6.0, and the third chromatography step can be performed in flow-through mode with polypeptides having a low or high isoelectric point.
Inventors:
Falkenstein, Robert
Weidants, Birgit
Giesel, Claudia
Gray Tanner, Civil
Grossman, Adelbert
Hesse, Friderike
Kramer, Brigitte
Marc Pompati
Schaubmar, Andreas
Nylor
Weidants, Birgit
Giesel, Claudia
Gray Tanner, Civil
Grossman, Adelbert
Hesse, Friderike
Kramer, Brigitte
Marc Pompati
Schaubmar, Andreas
Nylor
Application Number:
JP2010542579A
Publication Date:
July 05, 2016
Filing Date:
January 15, 2009
Export Citation:
Assignee:
F. HOFFMANN-LA ROCHE AKTIENGESELLSCHAFT
International Classes:
C07K1/16; C07K14/555; C07K14/61; C12N15/09; C12P21/02; G01N30/88
Domestic Patent References:
| JP7135992A | ||||
| JP7322887A | ||||
| JP2007533651A | ||||
| JP2008500273A |
Foreign References:
| WO2007041713A1 |
Other References:
Methods,(2004),Vol.32,p.300-312
Attorney, Agent or Firm:
Patent business corporation Tsukuni
Hajime Tsukuni
Toshio Miyake
Akio Shibata
Yasuhiro Oguni
Yoko Tanaka
Yoshinori Ikukawa
Hajime Tsukuni
Toshio Miyake
Akio Shibata
Yasuhiro Oguni
Yoko Tanaka
Yoshinori Ikukawa