Title:
SYSTEMS AND METHODS FOR MODEL-BASED qPCR
Document Type and Number:
Japanese Patent JP2016076242
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide a method for determining a cycle threshold for a polymerase chain reaction (PCR) amplification curve.SOLUTION: The method includes: receiving a data set for a plurality of biological samples for a PCR amplification reaction 10; fitting each amplification curve to a complementary modeled amplification curve to determine a best-fit set of parameters for a modeled efficiency curve and an associated amplification curve 20; and determining a cycle threshold value for each biological sample on the basis of a complementary relationship of the modeled efficiency curve to the modeled amplification curve 30.SELECTED DRAWING: Figure 1
Inventors:
WALLACE R GEORGE
Application Number:
JP2015231320A
Publication Date:
May 12, 2016
Filing Date:
November 27, 2015
Export Citation:
Assignee:
LIFE TECHNOLOGIES CORP
International Classes:
G16B25/20; G16B40/10
Domestic Patent References:
| JP2007095070A | 2007-04-12 | |||
| JP2007128483A | 2007-05-24 | |||
| JP2001314194A | 2001-11-13 | |||
| JP2007236219A | 2007-09-20 | |||
| JP2008521104A | 2008-06-19 | |||
| JP2005516630A | 2005-06-09 | |||
| JP2013504987A | 2013-02-07 |
Foreign References:
| US20100070190A1 | 2010-03-18 |
Other References:
ROBERT G RUTLEDGE ET AL: "A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capaci", BMC BIOTECHNOLOGY, vol. 8, no. 47, JPN6015003217, 8 May 2008 (2008-05-08), pages 1 - 28, ISSN: 0003649492
Attorney, Agent or Firm:
Hatsushi Shimizu
Masao Haruna
Hirotaka Yamaguchi
Toshi Gobe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Masato Ozeki
Yoshihiro Igarashi
Kazuya Kawamoto
Masao Haruna
Hirotaka Yamaguchi
Toshi Gobe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Masato Ozeki
Yoshihiro Igarashi
Kazuya Kawamoto