SOLUBLE POLYPEPTIDE-ISOLATION METHOD - NAT RES COUNCIL CANADA

Title:

SOLUBLE POLYPEPTIDE-ISOLATION METHOD

Document Type and Number:

Japanese Patent JP2014144010

Kind Code:

A

Abstract:

PROBLEM TO BE SOLVED: To provide a high-throughput screening method for identifying a polypeptide with improved biophysical properties (including solubility, high expression and/or stability (high refolding for thermal denaturation, high resistance to a chemical denaturant, high resistance to protease, especially to gastrointestinal protease such as trypsin, and the like)), especially an antibody fragment.SOLUTION: Using high throughput method for screening a polypeptide, a polypeptide (including monomeric human Vand human V) having desired biophysical properties such as solubility, stability, high expression property, monomer property, binding specificity and non-aggregability is identified. The method comprises the steps of: obtaining a phage display library; allowing infection of a bacterial layer with a library phage; and identifying a phage forming a plaque larger than the average on a layer of cell.

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Inventors:

TANHA JAMSHID

Application Number:

JP2014070227A

Publication Date:

August 14, 2014

Filing Date:

March 28, 2014

Export Citation:

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Assignee:

NAT RES COUNCIL CANADA

International Classes:

C12N15/09; A61K39/395; A61K45/00; A61P43/00; C07K16/00; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C40B30/04; C40B40/10

Domestic Patent References:

JP2002162398A	2002-06-07
JP2008536490A	2008-09-11
JP2002162398A	2002-06-07

Other References:

JPN6015020590; ＧｅｎｅＶｏｌ．１６０, 1995, ｐｐ．２６９-２７２
JPN6013048873; Ａｎｇｅｗ．　Ｃｈｅｍ．　Ｉｎｔ．　Ｅｄ．Ｖｏｌ．４１, 2002, ｐｐ．４４０２-４４２５
JPN6013048876; Ｂｉｏｔｅｃｈｎｏｌｏｇｙ　ＡｄｖａｎｃｅｓＶｏｌ．２１, 2003, ｐｐ．５９９-６３７

Attorney, Agent or Firm:

Patent business corporation 3 Edakuni [Hajime] patent firm
Eiji Saegusa
Mutsuko Nakano
Wood Masahito
Nagafumi Kawai

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