Title:
【発明の名称】イソペニシリンN合成酵素をコ-ドしているDNA化合物および組換えDNA発現ベクタ-
Document Type and Number:
Japanese Patent JPH0787788
Kind Code:
B2
Abstract:
The present invention comprises novel DNA compounds that encode isopenicillin N synthetase and also comprises related methods, transformants, and polypeptides. The novel isopenicillin N synthetase-encoding DNA, together with its associated transcriptional and translational activating sequence, was isolated from Cephalosporium acremonium and cloned into an E. coli cloning vector. The isopenicillin N synthetase-encoding DNA has been used to construct novel E. coli expression vectors that drive expression of a stable, active, and novel isopenicillin N synthetase in E. coli. The intact C. acremonium isopenicillin N synthetase-encoding DNA and associated transcriptional and translational activating sequence have also been used to construct C. acremonium expression vectors that drive expression of the isopenicillin N synthetase in C. acremonium. The C. acremonium transcriptional and translational activating sequence has further been fused to a hygromycin phosphotransferase-encoding DNA segment and placed onto C. acremonium expression vectors. Useful derivatives of the novel compounds and vectors are also described.
Inventors:
Thomas Dominique Ingoria
Stefan Weat Queener
Suren Mary Samson
Paul Luther Skaterad
Stefan Weat Queener
Suren Mary Samson
Paul Luther Skaterad
Application Number:
JP9317886A
Publication Date:
September 27, 1995
Filing Date:
April 21, 1986
Export Citation:
Assignee:
Lee-Lee Lili-and Company-
International Classes:
C07H21/04; C12N1/00; C12N1/21; C12N9/00; C12N15/00; C12N15/09; C12N15/69; C12N15/70; C12N15/74; C12N15/80; C12P37/00; C12N15/52; C12R1/01; C12R1/19; C12R1/365; C12R1/465; C12R1/75; C12R1/82; (IPC1-7): C12N15/09; C12N9/00; C12P37/00
Other References:
Ｓｃｉｅｎｃｅ，２２４（１９８４）Ｐ．６１０−６１２
Attorney, Agent or Firm:
Aoyama Ryo (1 person outside)