Title:
【発明の名称】核酸配列マニュピレーション法
Document Type and Number:
Japanese Patent JPH07502405
Kind Code:
A
Abstract:
A method of effecting a manipulation of a nucleic acid sequence comprises (a) providing a solid support system having bonded thereto a single stranded oligonucleotide complementary to a specific sequence on a target nucleic acid longer than said oligonucleotide, (b) adding a source of single stranded target nucleic acid to the solid support system, (c) hybridising the target nucleic acid to the oligonucleotide, and (d) effecting the manipulation on the hybridised target nucleic acid. The manipulation may for example be a copying or amplification of the target nucleic acid sequence. In a preferred embodiment, the support is provided in a flow-through vessel which facilitates washing of the support to remove impurities and leave a "clean" sample of target nucleic acid on which the manipulation may be carried out. The support preferably has a siloxane matrix to which the oligonucleotide is bound. This provides a stable linkage between the oligonucleotide and the support. The method is particularly useful for analysis of medical samples.
Inventors:
Steven, Minter
Application Number:
JP51127893A
Publication Date:
March 16, 1995
Filing Date:
December 23, 1992
Export Citation:
Assignee:
Tepnel Medical Limited
International Classes:
B01D15/32; B01J20/26; B01J20/32; B01L3/00; B01L7/00; C07H21/00; C12M1/00; C12Q1/68; C12Q1/70; C12N15/09; B01J20/283; B01J20/286; C12R1/145; C12R1/19; C12R1/35; C12R1/42; C12R1/445; C12R1/92; (IPC1-7): C12Q1/68; B01J20/26; C07H21/00; C12M1/00; C12N15/09; C12Q1/70
Attorney, Agent or Firm:
Aoyama Ryo (1 person outside)