PURPOSE: In determining the desired substance existing in a specimen with an ammonia formation system, to determine accurately, by pretreating the specimen with a specific enzymatic system, converting ammonia existing in the specimen into glutamic acid and water.
CONSTITUTION: A specimen such as urea, blood, etc. is blended with glutamic dehydrogenase, 2-oxoglutaric acid, a small amount of reduction type nicotinamide adenine dinucleotide phosphate (NADPH for short), and an enzyme and substrate to reduce nicotinamide adnine dinucleotide phosphate (NADP+ for short). Ammonia already existing in the specimen is converted into glutamic acid and water, and NADP+ prepared in the conversion is recovered into NADPH. Ammonia existing in the specimen is removed by the operation, and the desired substance existing in the specimen is determined using an ammonia formation system.
FUJITA TAKESHI
SUZUKI YASUO
KOUKAWARA ISAMU
FUJII KATSUMI
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