Title:
【発明の名称】アジポイル―7―ADACを製造するための新規なバイオプロセス
Document Type and Number:
Japanese Patent JP2655790
Kind Code:
B2
Abstract:
Important intermediates for preparing cephalosporin antibiotics, 7-amino-cephalosporanic acid (7-ACA) and 7-aminodeacetylcephalosporanic acid (7-ADAC), are prepared by a novel bioprocess in which a transformed Penicillium chrysogenum strain is cultured in the presence of an adipate feedstock to produce adipoyl-6-APA (6-amino penicillanic acid); followed by the in situ expression of the following genes with which the P. chrysogenum has been transformed: 1) an expandase gene, e.g., from Cephalosporium acremonium, whose expression product converts the adipoyl-6-APA by ring expansion to adipoyl-7-ADCA; 2) an hydroxylase gene whose expression product converts the 3-methyl side chain of adipoyl-7-ADCA to 3-hydroxymethyl, to give the first product, adipoyl-7-aminodeacetylcephalosporanic acid (7-ADAC); and 3) an acetyltransferase gene whose expression product converts the 3-hydroxymethyl side chain to the 3-acetyloxymethyl side chain of adipoyl-7-ACA. The final product, 7-ACA, is then prepared by cleavage of the adipoyl side chain using an adipoyl acylase. The entire synthesis, accordingly, is carried out using bioprocesses, and is efficient and economical.
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Inventors:
MAIKERU JEI KONDAA
ANSONII MAIKERU SUTEPAN
RORIRII KUROOFUOODO
JON EI RANBOSETSUKU
FUIRISU SHII MAKADA
KURISUTOFUAA DEII RIIBUSU
ANSONII MAIKERU SUTEPAN
RORIRII KUROOFUOODO
JON EI RANBOSETSUKU
FUIRISU SHII MAKADA
KURISUTOFUAA DEII RIIBUSU
Application Number:
JP31919492A
Publication Date:
September 24, 1997
Filing Date:
October 15, 1992
Export Citation:
Assignee:
GISUTO BUROKADESU NV
International Classes:
C07D501/20; C12N1/15; C12N9/00; C12N9/02; C12N9/10; C12N1/19; C12N15/09; C12N15/52; C12N15/54; C12P35/00; C12P35/06; C12P37/04; C12R1/82; C12P; (IPC1-7): C12P35/00; C12N1/19; C12N15/09
Domestic Patent References:
| JP61152286A | ||||
| JP62500350A |
Other References:
【文献】欧州公開437378(EP,A)
【文献】欧州公開450758(EP,A)
【文献】欧州公開281391(EP,A1)
【文献】欧州公開450758(EP,A)
【文献】欧州公開281391(EP,A1)
Attorney, Agent or Firm:
Yoshio Kawaguchi (3 outside)