Title:
METHOD OF PRODUCING RECOMBINANT HIGH MOLECULAR WEIGHT vWF IN CELL CULTURE
Document Type and Number:
Japanese Patent JP2017217004
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide a method of producing recombinant high molecular weight vWF in a cell culture.SOLUTION: Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimeric WF, having a high specific activity, and ADAMTS13 having a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities.SELECTED DRAWING: Figure 1A
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Inventors:
GRILLBERGER LEOPOLD
LEITER MANFRED
WOLFGANG MUNDT
LEITER MANFRED
WOLFGANG MUNDT
Application Number:
JP2017149409A
Publication Date:
December 14, 2017
Filing Date:
August 01, 2017
Export Citation:
Assignee:
BAXALTA INC
BAXALTA GMBH
BAXALTA GMBH
International Classes:
C12P21/02; A61K38/36; A61P7/04; C07K14/745; C07K14/755; C12N1/00; C12N5/10
Attorney, Agent or Firm:
Hidesaku Yamamoto
Natsuki Morishita
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto
Natsuki Morishita
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto