クモの小吐糸管絹糸蛋白質をコードするｃＤＮＡ - ユニバーシティーオブワイオミング

Title:

クモの小吐糸管絹糸蛋白質をコードするｃＤＮＡ

Document Type and Number:

Japanese Patent JPH09510349

Kind Code:

A

Abstract:

cDNA clones encoding minor ampullate spidroin proteins (MiSP) are described. The translated amino acid sequence of the cloned cDNA shows that the MiSPs have a structure which exhibits an amino proximal nonrepetitive region, a repetitive portion and a carboxy-proximal nonrepetitive portion. The repetitive portion of the sequence is describable by a generic repeat formula. Comparison of the amino acid sequences derived from the translation with the sequences of short peptides obtained from solubilized minor ampullate spider silk suggests that the nonrepetitive portions of the protein are cleaved from the protein during secretion from the cells synthesizing the spidroins. This comparison also suggests that the minor ampullate spider silk is composed of at least three polypeptides.

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Inventors:

コルギン，マーク
ルイス，ランドルフブイ．

Application Number:

JP52412295A

Publication Date:

October 21, 1997

Filing Date:

March 14, 1995

Export Citation:

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Assignee:

ユニバーシティーオブワイオミング

International Classes:

C07K14/435; C12N1/21; C12N15/12; C12P21/02; D01F4/00; C12N15/09; C12R1/19; (IPC1-7): C12N15/09; C07K14/435; C12N1/21; C12P21/02; D01F4/00

Attorney, Agent or Firm:

細田芳徳

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