Title:
酢酸菌の増殖促進機能に関与する遺伝子、該遺伝子を用いて育種された酢酸菌、及び該酢酸菌を用いた食酢の製造方法
Document Type and Number:
Japanese Patent JP4528270
Kind Code:
B2
Abstract:
The present invention is to obtain a novel gene that encodes a protein having a function of promoting acetic acid bacterial growth in the presence of a high concentration of acetic acid; to enhance acetic acid bacteria's function of promoting growth and thereby to develop acetic acid bacteria with an improved fermentation ability in the presence of a high concentration of acetic acid; and to provide a method for efficiently producing vinegar that contains a high concentration of acetic acid in a short time using the acetic acid bacterium. A novel gene having a function of improving the function of promoting growth at a practical level was cloned from acetic acid bacteria for practical use, belonging to the genus Gluconacetobacter, by a method comprising exposing acetic acid bacterial lysate to the presence of a high concentration of acetic acid; obtaining a gene of a protein that was not insolubilized but remained soluble; and thereby obtaining a gene having a function of promoting acetic acid bacterial growth in the presence of acetic acid. When cultured in the presence of acetic acid and ethanol, a transformant of acetic acid bacteria transformed with the gene was confirmed to have a significant effect of promoting growth.
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Inventors:
Kondo Mio
Application Number:
JP2006058533A
Publication Date:
August 18, 2010
Filing Date:
March 03, 2006
Export Citation:
Assignee:
Mizkan Group Headquarters
International Classes:
C12N15/09; C07K14/195; C12J1/04; C12N1/21
Foreign References:
| WO2003078635A1 | ||||
| WO2004081216A1 | ||||
| WO2005001095A1 |
Other References:
Nat. Biotechnol. (2005) vol.23, no.2, p.195-200
Database UniprotKB [online] , Accessin No. Q5FSI5, 10-MAY-2005 uploaded, Prust, C. et al., Definition: Peptidyl-prolyl cis-trans isomerase
Database UniprotKB [online] , Accessin No. Q5FSI5,
Attorney, Agent or Firm:
Masaki Hirota
Seiji Ozawa
Large High Toshiko
High Kazuya Tsu
Makoto Horiuchi
Seiji Ozawa
Large High Toshiko
High Kazuya Tsu
Makoto Horiuchi