Title:
A manufacturing method by the fermentation of sulfur content amino acid
Document Type and Number:
Japanese Patent JP6053691
Kind Code:
B2
Abstract:
Fermentative production of sulfur-containing amino acids comprising L-methionine, L-cysteine, L-cystine, L-homocysteine or L-homocystine, comprises: (a) providing a microorganism from a family of Enterobacteriaceae or Corynebacteriaceae, having increased thiosulfate sulfur-transferase activity compared to their respective parent strain; (b) fermenting the microorganism in a medium, which contains an inorganic sulfur source to obtain a fermentation broth; and (c) enriching the sulfur-containing amino acid in the fermentation broth. Fermentative production of sulfur-containing amino acids comprising L-methionine, L-cysteine, L-cystine, L-homocysteine or L-homocystine, comprises: (a) providing a microorganism from a family of Enterobacteriaceae or Corynebacteriaceae, having increased thiosulfate sulfur-transferase activity compared to their respective parent strain; (b) fermenting the microorganism in a medium, which contains an inorganic sulfur source comprising dithiosulfuric acid salt or a mixture of a salt of dithiosulfuric acid and a salt of sulfuric acid, to obtain a fermentation broth; and (c) enriching the sulfur-containing amino acid in the fermentation broth. Independent claims are included for a microorganism comprising Escherichia colihaving increased activity and/or expression of the aspartate and mitigation or deletion of the regulatory protein McbR compared to the parental strain, or Corynebacterium glutamicumhaving increased activity and/or expression of the aspartate and attenuation or deletion of the metJ regulatory protein compared to the parental strain, where the microorganism excretes or produces L-methionine, and the microorganism overexpresses a gene coding for a polypeptide having increased thiosulfate sulfur-transferase activity compared to parent strains.
Inventors:
Frank schneider
Stella Mork
Brigitte batte
Stella Mork
Brigitte batte
Application Number:
JP2013549773A
Publication Date:
December 27, 2016
Filing Date:
January 12, 2012
Export Citation:
Assignee:
Evonik Degussa GmbH
International Classes:
C12P13/12; C12N1/21; C12N15/09
Domestic Patent References:
| JP2010539962A | ||||
| JP2009521939A |
Foreign References:
| WO2007135188A1 |
Other References:
The Open Microbiology Journal,2008年,vol.2,pp.18-28
Attorney, Agent or Firm:
Einzel Felix-Reinhard
Takuya Kuno
Takuya Kuno
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