Title:
MANUFACTURING METHOD OF FRESHNESS-MEASURING REAGENT KIT
Document Type and Number:
Japanese Patent JP2016101176
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To obtain a manufacturing method of a freshness-measuring reagent kit which has satisfactory storage stability at relatively high temperature.SOLUTION: A manufacturing method of a freshness-measuring reagent kit comprises: a process of drying a frozen object of first reagent solution under reduced pressure at the temperature of glass transition point temperature (Tg) or less; and a process of drying a frozen object of second reagent solution under reduced pressure at the temperature of glass transition point temperature (Tg) or less, in which the first reagent solution contains XOD, NP, enzyme protective agent and coloring agent, the second reagent contains XOD, NP, AP, enzyme protective agent and coloring agent, the enzyme protective agent consists of sucrose and/or gelatin, and the coloring agent consists of an agent that develops color conjugated with reaction in which Hx is decomposed into xanthine and urate by XOD.SELECTED DRAWING: Figure 3
Inventors:
SUZUKI TORU
HAMADA NAHOKO
PAVEENA SRIRANGSAN
KAWAI SEIJI
HAMADA NAHOKO
PAVEENA SRIRANGSAN
KAWAI SEIJI
Application Number:
JP2016028144A
Publication Date:
June 02, 2016
Filing Date:
February 17, 2016
Export Citation:
Assignee:
UNIV TOKYO MARINE SCI & TECH
International Classes:
C12N9/02; C12N9/10; C12Q1/28; C12Q1/48; G01N33/12
Domestic Patent References:
| JPH01289500A | 1989-11-21 | |||
| JPS6049800A | 1985-03-19 | |||
| JPH01279843A | 1989-11-10 | |||
| JP2007517888A | 2007-07-05 | |||
| JP2002512970A | 2002-05-08 | |||
| JPH02265984A | 1990-10-30 | |||
| JPH11504802A | 1999-05-11 | |||
| JP2000513940A | 2000-10-24 |
Attorney, Agent or Firm:
Noriaki Kubota