Title:
細胞内に存在するタンパク質又は当該タンパク質と相互作用する物質を分析する方法
Document Type and Number:
Japanese Patent JP4188229
Kind Code:
B2
Abstract:
Identifying or analyzing (M1) a protein occurring in cells or the cell surface, or a substance interacting with the protein, comprising: (a) contacting the cells with the substance; (b) purifying the cells; (c) irradiating the purified cells with laser to directly ionize the substance; and (d) analyzing the thus ionized substance by mass spectrometry (MS), is new. Independent claims are also included for: (1) screening (M2) a substance that interact with a protein on the cell surface or in cells and having a known activity, comprising: (a) bringing them into contact; (b) purifying the cells; (c) laser irradiation of the purified cells for direct ionization of the substance; and (d) analysis of the ionized substance by MS; and (2) screening (M3) cells or tissues expressing a protein that interacts with a substance having a known activity, comprising: (a) bringing them into contact; (b) purifying the tissues or cells; (c) laser irradiation of the purified tissues or cells for direct ionization; and (d) analysis of the ionized substance by MS, during which the mass spectra produced are compared with those obtained in a control run for judging the presence or absence of the protein based on the interaction.
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Inventors:
Akira Nakajima
Hideo Naoki
Yasuda Meiwa
Keiko Iwasa
Hideo Naoki
Yasuda Meiwa
Keiko Iwasa
Application Number:
JP2003505625A
Publication Date:
November 26, 2008
Filing Date:
June 19, 2002
Export Citation:
Assignee:
Suntory Limited
International Classes:
G01N33/483; C12Q1/02; C40B30/04; G01N27/62; G01N27/64; G01N30/72; G01N30/88; G01N33/50; G01N33/566; G01N33/68
Foreign References:
| WO1999045150A1 |
Attorney, Agent or Firm:
Kazuo Shamoto
Tadashi Masui
Yasushi Kobayashi
Akio Chiba
Hiroyuki Tomita
Tadahiko Kurita
Tadashi Masui
Yasushi Kobayashi
Akio Chiba
Hiroyuki Tomita
Tadahiko Kurita