Title:
METHODS FOR EXPRESSING POLYPEPTIDES USING MODIFIED NUCLEIC ACIDS
Document Type and Number:
Japanese Patent JP2018038408
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide methods for recombinantly producing polypeptides in cells.SOLUTION: The method of the invention comprises the steps of: culturing a cell comprising a nucleic acid encoding a polypeptide; and recovering the polypeptide from the cell or the culture medium, where each of the amino acid residues of the polypeptide is encoded by at least one codon, and different codons encoding the same amino acid residue are combined into one group, each of the codons in one group is defined by a specific usage frequency in the group, the total of the specific usage frequencies of all codons in one group is 100%, and the usage frequency of a codon in the nucleic acid encoding the polypeptide is almost the same as its specific usage frequency in its group.SELECTED DRAWING: None
Inventors:
STEFAN KLOSTERMANN
ERHARD KOPETZKI
URSULA SCHWARZ
ERHARD KOPETZKI
URSULA SCHWARZ
Application Number:
JP2017193965A
Publication Date:
March 15, 2018
Filing Date:
October 04, 2017
Export Citation:
Assignee:
HOFFMANN LA ROCHE
International Classes:
C12P21/02; C12N15/09
Domestic Patent References:
| JP2004503235A | 2004-02-05 |
Attorney, Agent or Firm:
Hatsushi Shimizu
Masao Haruna
Hirotaka Yamaguchi
Toshi Gobe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Hideki Kodera
Masato Ozeki
Yoshihiro Igarashi
Kazuya Kawamoto
Masao Haruna
Hirotaka Yamaguchi
Toshi Gobe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Hideki Kodera
Masato Ozeki
Yoshihiro Igarashi
Kazuya Kawamoto
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