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Title:
変異型D-アミノトランスフェラーゼおよびこれを用いた光学活性グルタミン酸誘導体の製造方法
Document Type and Number:
Japanese Patent JP4544154
Kind Code:
B2
Abstract:
A D-aminotransferase can be modified so as to efficiently produce (2R, 4R) -monatin having high sweetness intensity from 4- (indol-3-ylmethyl) -4-hydroxy-2-oxoglutaric acid by substituting an amino acid at least at one of positions (positions 100, 180 to 183, 243 and 244) involved in efficiently producing the (2R, 4R) -monatin in an amino acid sequence of a wild-type D-aminotransferase represented in SEQ ID NO:2.

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Inventors:
Masakazu Sugiyama
Watanabe Otohiko
Tashimi Kashiwagi
Eiichiro Suzuki
Application Number:
JP2005502362A
Publication Date:
September 15, 2010
Filing Date:
December 09, 2003
Export Citation:
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Assignee:
AJINOMOTO CO.,LTD.
International Classes:
C12N15/09; C07K14/32; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/10; C12P13/04; C12P17/10; C12P21/02; G01N33/68
Foreign References:
EP0736604A21996-10-09
Attorney, Agent or Firm:
Hiroaki Sakai
Tadashi Terasaki
Takuya Kashima



 
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