Title:
A primer for Maiko bacterium Avium detection, a probe, and a detecting method of Maiko bacterium Avium using this
Document Type and Number:
Japanese Patent JP6166806
Kind Code:
B2
Abstract:
The object of the present invention is to provide a novel primer for use in the detection of Mycobacterium avium ( M. avium ), and a method for detection of M. avium conveniently, rapidly and with high precision by using the primer. More specifically, disclosed are an oligonucleotide which comprises a part or an entire of the nucleotide sequence selected from SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:130, SEQ ID NO:131, SEQ ID NO:132, SEQ ID NO:133, SEQ ID NO:134, SEQ ID NO:135 and SEQ ID NO:136, or a part or an entire of the sequence complementary to the nucleotide sequence, and which is capable of hybridizing with the nucleotide sequence for a Mycobacterium avium gene; a primer and a probe for use in the detection of M. avium , which comprises the oligonucleoride; and a method for detection of M. avium using the primer and/or probe.
Inventors:
Yuichi Ishikawa
Application Number:
JP2016014806A
Publication Date:
July 19, 2017
Filing Date:
January 28, 2016
Export Citation:
Assignee:
Wako Pure Chemical Industries, Ltd.
International Classes:
C12N15/09; C12Q1/68
Foreign References:
| WO2006121134A1 |
Other References:
CHEN Z.H. et al.,Identification and differentiation of Mycobacterium avium and M. intracellulare by PCR,J. Clin. Microbiol.,1996年 5月,Vol.34,No.5,pp.1267-1269
SRITHARAN V. et al.,Specificity of diagnostic PCR amplification for M. avium using the probe pMAV22,Mol. Cell. Probes,1995年 2月,Vol.9,No.1,pp.71-74
FROTHINGHAM R. et al.,Sequence-based differentiation of strains in the Mycobacterium avium complex,J. Bacteriol.,1993年 5月,Vol.175,No.10,pp.2818-2825
SRITHARAN V. et al.,Specificity of diagnostic PCR amplification for M. avium using the probe pMAV22,Mol. Cell. Probes,1995年 2月,Vol.9,No.1,pp.71-74
FROTHINGHAM R. et al.,Sequence-based differentiation of strains in the Mycobacterium avium complex,J. Bacteriol.,1993年 5月,Vol.175,No.10,pp.2818-2825
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