The random genome insertion and the deficiency tool for Rhodococcus bacteria

Title:

The random genome insertion and the deficiency tool for Rhodococcus bacteria

Document Type and Number:

Japanese Patent JP6067204

Kind Code:

B2

Abstract:

PROBLEM TO BE SOLVED: To provide a method for causing random deletion of functions of an intracellular DNA in a genus Rhodococcus bacterium; and to provide the genus Rhodococcus bacterium strain prepared by this method.SOLUTION: There are provided: the method for inserting a straight-chain DNA into the genome of the bacterium by transforming this bacterium belonging to genus Rhodococcus by using a straight-chain DNA fragment including an arbitrary sequence (preferably, one or more selective marker genes); the method for randomly losing the functions of the intracellular DNA of the bacterium; and a transformant prepared by this method.

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Inventors:

Fujio Yu
Miki Wakamatsu

Application Number:

JP2010096845A

Publication Date:

January 25, 2017

Filing Date:

April 20, 2010

Export Citation:

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Assignee:

Mitsubishi Rayon Co., Ltd.

International Classes:

C12N15/09; C12N1/21

Domestic Patent References:

JP2007228894A
JP2008067629A
JP2008259451A

Other References:

Mol. Microbiol., 1991, vol.5, no.9, p.2115-24

Attorney, Agent or Firm:

Reiko Tanaka
Yuko Matsuniya
Umeda Shinsuke
Hiroshi Morita

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