Title:
ADAPTER MOLECULE CAPABLE OF REVERSIBLY EQUIPPING A FUSION PROTEIN CARRYING AN OLIGOHISTIDINE AFFINITY TAG WITH A FURTHER AFFINITY TAG AND METHODS OF USING THE SAME
Document Type and Number:
WIPO Patent Application WO/2013/160453
Kind Code:
A3
Abstract:
Disclosed is a bifunctional adapter molecule comprising two binding moieties A and B, the adapter molecule being capable of reversibly equipping a fusion protein carrying an oligohistidine affinity tag with a further affinity tag, wherein the binding moiety A comprises at least two chelating groups K, wherein each chelating group is capable of binding to a transition metal ion, thereby rendering moiety A capable of binding to an oligohistidine affinity tag, and the binding moiety B is an affinity tag other than an oligohistidine tag.
Inventors:
SCHMIDT THOMAS (DE)
KOTH CHRISTOPHER (US)
KOTH CHRISTOPHER (US)
Application Number:
PCT/EP2013/058745
Publication Date:
February 20, 2014
Filing Date:
April 26, 2013
Export Citation:
Assignee:
IBA GMBH (DE)
International Classes:
C07K1/22; C07K1/13; C07K14/36; G01N33/68
Domestic Patent References:
| WO2000047548A1 | 2000-08-17 |
Foreign References:
| US6103493A | 2000-08-15 |
Other References:
RONALD K. JUNE ET AL: "Synthesis of a pH-Sensitive Nitrilotriacetic Linker to Peptide Transduction Domains To Enable Intracellular Delivery of Histidine Imidazole Ring-Containing Macromolecules", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 132, no. 31, 11 August 2010 (2010-08-11), pages 10680 - 10682, XP055075282, ISSN: 0002-7863, DOI: 10.1021/ja1040418
Attorney, Agent or Firm:
SCHIWECK, Wolfram et al. (European Patent AttorneysLandsberger Str. 98, Munich, DE)
Download PDF: