Title:
ADAPTER SUITABLE FOR ULTRA-TRACE DNA SEQUENCING, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2017/202389
Kind Code:
A1
Abstract:
An adapter suitable for ultra-trace DNA sequencing, and an application thereof. From the 5' end to the 3' end the adapter comprises in turn a Tag sequence, a PolyN sequence, a first hairpin complementary sequence, a first hairpin structure sequence, dUTP, a second hairpin structure sequence, and a second hairpin complementary sequence; the second hairpin complementary sequence is a reverse complementary sequence of the first hairpin complementary sequence; and the 5' end of the adapter has a phosphorylation tag. Hairpin structures are designed in the present adapter such that, when in use, the adapter self-forms the hairpin structures and is ligated to DNA to be sequenced, effectively preventing the problem of low ligation efficiency caused by self-ligation of the adapter when being ligated to DNA to be sequenced. The present adapter is particularly suitable for use in ultra-trace DNA library construction and sequencing, and for laying the foundation for accurate detection of ctDNA.
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Inventors:
ZHANG XIAONI (CN)
LIU MING (CN)
ZHONG GUOLIN (CN)
XU MINGYAN (CN)
LIU MING (CN)
ZHONG GUOLIN (CN)
XU MINGYAN (CN)
Application Number:
PCT/CN2017/086330
Publication Date:
November 30, 2017
Filing Date:
May 27, 2017
Export Citation:
Assignee:
HAPLOX BIOTECHNOLOGY (SHENZHEN) CO LTD (CN)
International Classes:
C12N15/11; C12N15/10; C12Q1/68; C40B50/06
Domestic Patent References:
| WO2009082488A2 | 2009-07-02 |
Foreign References:
| CN106047998A | 2016-10-26 | |||
| CN105442054A | 2016-03-30 | |||
| CN105525357A | 2016-04-27 | |||
| CN105154444A | 2015-12-16 | |||
| US6235502B1 | 2001-05-22 |
Attorney, Agent or Firm:
DHC IP ATTORNEYS (CN)
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