Field of the invention

This invention is in the field of the control of fungal infection in plants.

Description of the prior art

The effect of compounds which interfere with polyamine metabolism on the growth of the fungus Botrvtis cinerea has been investigated, Smith ___ __±. , J- Gen. Microbiol. 136, 985 (1990). This work demonstrated that difluoromethylornithine, (DFMO) inhibition of fungal growth could be reversed with the addition of putrescine, cadaverine, spermidine and sper ine. "Butenedia ine" itself caused some inhibition of fungal growth but significantly reversed the inhibitory effect of DFMO. Summary of the invention

We have now discovered that certain alicyclic diamines exhibit useful antifungal activity.

Accordingly, the invention provides the use of a compound having the general formula (1):

wherein R ¹ , R ² , R ³ and R ⁴ which may be the same or different represent hydrogen atoms or alk l groups of 1-6 carbon atoms or NR R ² and/or NR ³ R ⁴ together represent a heterocyclic group having 5 or 6 ring atoms, the dashed line represents a double bond, W represents a saturated or ethylenically unsaturated divalent hydrocarbyl group required to complete a cycloalkane, cycloalkene or cycloalkadiene ring of 3 to 6 ring atoms, preferably 4 to 6 ring atoms, and from 0 to 2 double bonds, in the form of a free base or acid addition salt tolerable to plants, as a plant fungicide.

Description of the Preferred Embodiments

One class of preferred compounds of formula (1) is those wherein W completes a cycloalkane or cycloalkene ring of 4 to 6 ring atoms. W represents preferably a group having the formula - (CH ₂ ) _n - wherein n is from 2 to 4 or a group -CH -CHyR ⁵ ₌₌ CHyR ⁶ -CH ₂ - wherein y is 0 or 1 and when y is 0 the dashed line represents a double bond and R- and R" which may be the same or different represent hydrogen atoms or alkyl groups of 1 to 4 carbon atoms, especially methyl groups. R ¹ , R ² , R ³ and R ⁴ are preferably alkyl groups of 1 to 4 carbon atoms, especially methyl, or hydrogen atoms.

One particularly preferred class of compounds for use as a plant fungicide is trans -N.N.N' ,N'-tetramethyl or -tetraethyl-1 ,2-bis(aminomethyl)cyclobutane [better named trans-1 ,2-bis(methylaminomethyl or ethylaminomethyDcyclobutane] and also 1 ,2-bis(methylaminomethyl or ethylaminomethyl)cyclohexa-l ,4-diene.

Others are the primary amines, (R ¹ , R ² , R ³ and R ⁴ are all hydrogen). Within this group, particularly preferred compounds are 1 ,2-bis(aminomethyl)-4,5-dimethylcyclohexa-l ,4-diene and 1 ,2-dimethyl-4,5-bis(aminomethyl)cyclohex-l-ene, of which the trans isomer is more preferred than the ~1_ isomer.

Preferred heterocyclic groups NR^R ² and/or NR ³ R ⁴ are pyrrolidino, piperidino, 4-methylpiperidino and morpholino. All these diamines can form salts with mineral acids or organic adds. Preferred mineral acids are HC1 , HBr, HNO3, H2SO4, H3PO4. Preferred organic acids are propionic, benzoic, formic, acetic, trifluoroacetic, aleic, fumaric, succinic, alonic, tartaric, ascorbic, citric, oxalic, glyoxalic, alkyl or anyl sulphonic. These salts may be prepared by standard techniques and procedures well known in the art.

The compounds of the invention may be prepared by conventional techniques in organic chemistry and/or techniques analogous to those of the Examples herein, e.g. substituting pyrrolidine or morpholine for aliphatic amines.

The diamine compounds of the invention also exhibit antifungal activity when prepared in the form of their acid salts and such salts may be used as fungicides according to this invention. The compounds of the invention are particularly useful fungicides against fungi of the genera Erysiphe and Podosphaera. These fungi cause mildew in plants. Thus the compounds of the invention are particularly useful mildewicides. This invention also provides fungicidal compositions comprising the diamine compound together with a suitable diluent or carrier. Such diluents or carriers must not be phytotoxic to the plant materials. Suitable diluents or carriers include water and organic solvents. Preferably the concentration of diamine is between 0.001 to 0.1 molar. Thus, the diamine can be dispersed on a finely-divided solid carrier to form a dusting powder. Also, the amine can be dispersed in water, typically with the aid of a wetting agent, and the resulting aqueous dispersion employed as a spray. In other procedures the amine can be employed as a constituent of organic liquid compositions, oil-in-water and water-in-oil emulsions or dispersions, with or without the addition of wetting, dispersing or emulsifying agents.

Suitable adjuvants of the foregoing type are well known to those skilled in the art. The choice of such diluents, carriers and wetting agents (if required) will vary, depending upon the type of composition required for specific purposes and may be different for compositions intended to treat fields of crops or those compositions used for treating individual plants. The choice of such diluents or carriers and wetting agents will however be apparant to those skilled in the art. The methods of applying the solid or liquid fungicidal formulations are similarly well known.

The present invention also provides a method for the fungicidal treatment of plant material in either a preventative or curative mode using antifungal diamine compounds as described hereinbefore. The treatment may be applied to growing or harvested plant materials. If the plant material is growing, then the plants may be treated before they are infected by the fungi. This can be carried out by either treating the whole plant (e.g. by spraying it with a solution/emulsion or suspension on the antifungal compounds) or on specific parts of the plant, e.g. the leaves, stems, fruits or even seeds prior to planting. Treatment of the soil 1s another alternative since the antifungal compounds of the invention are systemic in their mode of action. Plants which have come into contact with fungi and thus already infected may be treated locally at site of infection or the whole plant may be treated.

The present invention also includes treatment of harvested plant parts for the prevention or control of fungal diseases. For this, various ways of carrying out the treatment can be employed. These will be well known to those skilled in the art, for example, treatment can be to the harvested plant itself by for example, dipping the plant part into a solution of the antifungal agent, or by impregnating fungicide into the wrapper, carton, crate, etc., in which the plant will be transported. Alternatively, the harvested plant material may be fumigated with the fungicide in a special room, car or tank.

A typical solid composition may be formulated by dry milling the active compound with BARDEN clay. This solid formulation or dust can contain the active compounds in amounts of from about 1 to about 25 percent by weight or more if desired. The dust is suitable for application to cereal seeds prior to planting.

A typical liquid composition is formulated by dissolving the active compound in a mixture of water and isopropanol (80:20 water/isopropanol ratios) containing a surfactant. This liquid formulation can contain the active compound in amounts of from about 15 to about 40 percent by weight or more if desired. The

aqueous formulation is suitable for application to cereal foliage or application as a seed drench, after suitable dilution with water.

The concentration of the active compounds in solid or liquid compositions is dependent upon the use, but for agricultural purposes generally is from about 1 to about 20 percent by weight or more. Concentrations from about 5 to about 10 percent by weight are often employed. In compositions to be employed as concentrates, the active compound can be present in a concentration from about 15 to about 50 weight percent, preferably 20 weight percent. The compositions containing the active compounds can also contain other compatible additives, for example when used 1n an agricultural context, phytotoxicants, plant growth regulants, pesticides, film-forming anti- transplrants, other fungicides and the like which are suitable for application to agricultural, horticultural, forestry and amenity crops. The present compositions can be applied to plants and crops by the use of power dusters, boom and hand sprayers, spray dusters and by other conventional means. The compositions can also be applied from airplanes as a dust spray since the ingredients are effective at very low application rates.

The exact rate to be applied is dependent not only on the specific amine being applied, but also on the particular treatment desired (e.g. animals, seeds, soil, or foliage) the particular crop being treated, climatic conditions, severity of any infection and the like. Thus, it is also to be understood that all of the active compounds of the present invention and compositions containing the same may not be equally effective at similar concentrations or against the same fungal species.

In foliar treatments, the active compounds of the present invention are usually applied at an approximate rate of from about 50 to 500 g/ha, a rate of from about 100 to 400 g/ha being preferred and a rate of from about 100 to about 350 g/ha being particularly preferred.

In seed treatments, the active compounds of the present invention are usually applied at an approximate rate of from about 60 to about 250 g per 100 kg seed, a rate of from about 100 g to about 200 g per 100 kg seed being preferred and a rate of from about 140 to about 180 g per 100 kg seed being particularly preferred. Seeds may be treated prior to planting and this may be carried out among other methods by fumigation.

In soil treatments, the active compounds of the present invention are usually applied at an approximate rate of from about 50 to about 350 g/ha, a rate of from about 100 to about 300 g/ha being preferred and a rate of from about 200 to about 280 g/ha being particularly preferred.

There are also provided fungicidal compositions comprising at least one diamine compound of formula (1) together with a suitable carrier, diluent and preferably a wetting agent. Such carriers, diluents and wetting agents must not be phytotoxic to plant materials when used in an agricultural context when the diamine is present at a fungicidal concentration. Suitable diluents and carriers include water and organic solvents, wherein the total concentration of the diamine compound or compounds is between 0.001 to 0.1 molar.

Many compounds of formula (1) above are novel βer e. Thus, the current invention extends to all such compounds, in particular those of the general formula (1) above, wherein R ¹ , R ² , R ³ and R ⁴ which may be the same or different represent hydrogen atoms or alkyl groups of 1-6, preferably 1-4, carbon atoms, or NR^ ² and/or NR ³ R ⁴ together represent a heterocyclic group having 5 or 6 ring atoms and W represents a group of formula -(CH ₂ ) _n - wherein n is 2 or 3 (thus completing a cyclobutane, cyclobut-1-ene, cyclopentane or cyclopent-1-ene ring) or of formula -CH ₂ -CHvR ⁵ —CHvR ⁶ -CH ₂ - wherein y is 0 or 1 and when y is 0 the dashed line represents a double bond, the sum of x and y is 1 or 2 (whereby W thus completes a cyclohexene or cyclohexadiene ring) and R^ and R^, which may be the same or different, represent hydrogen atoms or alkyl groups of 1 to 4 carbon atoms, in the form of the free base or as an acid

addi tion sal t tol erabl e to pl ants .

A parti cul arly preferred compound of the above formul a i s irani.-l , 2-di methyl -4 , 5-bi s(ami nomethyl )cyclohex-l -ene and i ts sai d sal ts .

EXAMPLES

This invention is illustrated by way of the following Examples. "Tween", "Celite", "Mistral" and "Bayfidan" are Registered Trade Marks.

Example 1 : Synthesis of 1.2-bis(aminomethyl)cyclopent-l-ene dihvdrochloride.

A. 1 ,2-Bis(hvdroxymethyl )cvclopent-l-ene

The title compound is known from D. Butina and F. Sondheimer, Synthesis 543-545 (1980). It was synthesised in 22% overall yield in three steps from pimelic acid (heptane-1 ,7-dioic acid) following the procedure of that paper. N.m.r: δH (90MHz, CDCI3) 4.21 (s,4H), 3.51 (bs,2H), 2.47 (t,4H), 1.83 ppm (m,2H).

B. 1.2-Bis(aminomethy1)cvclopent-1-ene dihvdrochloride

To a solution of hydrazoic acid (1.25M) 1n benzene (19ml) was added 1 ,2-bis(hydroxymethyl)cyclopent-l-ene (1.28g, O.Olmol) in dry THF (20ml),and diisopropyl azodicarboxylate (2.52g, 0.025mol) in dry THF (10ml). To this stirred solution was added triphenylphosphine (10.48g, 0.05mol) in dry THF (35ml) over 1 hour. The mixture was then stirred at 50 C for a further 16 hours, then water (5ml) added. The mixture was allowed to cool, then partitioned between hydrochloric acid (1M, 100ml) and CH ₂ C1 ₂ . (100ml) The aqueous layer was further washed with CH ₂ C1 ₂ (2 x 80ml), and the water removed in vacuo to give a brown solid, which was recrystallised twice (ethanol/acetone) to give the title compound, yield 21%. N.m.r:

SH (90 MHz, D ₂ 0) 4.05 (s,4H), 2.39 (t.4H), 1.89 ppm ⁽ m,2H ⁾

Example 2: Synthesis of 1.2-bis(aminomethyl) -4.5-dimethylcvclohexa-l .4-diene hydrochloride

A. Dimethyl 4.5-dimethylcvclohexa-l ,4-diene-l .2-dicarboxylate 2,3-dimethylbutadiene (9.84g, 0.12mol) and dimethyl acetylenedicarboxylate (14.2g, O.lmol) were stirred together in water (50ml) at 60 C for 24 hours. The emulsion was cooled to room temperature and filtered. The filtered solid was recrystallised from diethyl ether to give the title compound in 78% yield. N.m.r: δH (90 MHz, CDC1 ₃ ) 3.70 (s,6H), 1.71 ppm (s,6H)

B. 1 ,2-BisOivdroxymethyl)-4.5-dimethylcyc1ohexa-l .4-diene

To a solution of DIBAL in dichloromethane (1.0M, 45ml, 0.045mol) cooled to ice bath temperature under nitrogen was added dimethyl 4,5-dimethylcyclohexa-l ,4-diene-l ,2-dicarboxylate

(2.24g, O.Olmol) in dry dichloromethane (30ml) over 30 min. The resultant solution was stirred for a further 60 ins at this temperature after which methanol (10ml) was added. The mixture was allowed to come to room temperature, filtered through a

"Celite" pad, and the filtrate concentrated in vacuo to give an oil. Crystallisation from ethyl acetate/petroleum ether

(40°-60°C) gave the title compound in 35% yield. N.m.r: δH (90MHz, CD3OD) 4.15 (s,4H), 3.55 (bs,2H), 2.82 (s,4H), 1.69 ppm (s,6H)

C. Synthesis of 1.2-Bιs(aminomethyl) -4.5-dimethylcvclohexa-l .4-diene hydrochloride

The procedure of Example 1 was repeated using 1 ,2-bis(hydroxymethyl)-4,5-dimethyl-l ,4-cyclohexadiene as the starting diol. The title compound was obtained in 32% yield. N.m.r: δH (90 MHz, D ₂ 0) 3.98 (s,4H), 2.85 (s,4H), 1.62 ppm (s,6H)

Exampl e 3 : Synthesi s of 1 .2-bi s(ami nomethyl )cvclohex-l-ene di hvdrochlori de

A. Dimethylcvclohex-1-ene-l .2-dicarboxylate 3,4,5,6-tetrahydrophthalic anhydride (3.04g, 0.02mol) was disolved in methanol (25ml) and refluxed for 2 hours. The solution was then cooled to ice bath temperature and a solution of diazomethane in diethyl ether was added dropwise until a yellow colour persisted. The solution was then allowed to stand overnight, after which time the solvent was removed in vacuo to give the title compound in 93% yield. δH (90 MHz, CDC13) 3.66 (s,6H), 2.20 (s,4H), 1.66 ppm (s,6H)

B. 1.2-Bis(aminomethyl)cvclohex-l-ene dihvdrochloride The procedure for conversion of the above ester into the title compound was as described in Example 2 above. The title compound was produced in 12% overall yield. N.m.r: δH (90 MHz, D ₂ 0) 4.00 (s,4H), 2.15 (m,4H), 1.60 ppm (m,4H).

Example 4: Synthesis of trans-1.2-dimethyl-4.5-bis ⁽ aminomethyl)cvclohex-l-ene

A. Trans-4.5-bis(hvdroxymethyl)-l .2-dimethylcvclohex-l-ene

To a suspension of lithium alumium hydride (1.52g, 0.04mol) in dry THF (60ml) at 0°C was added trans-dimethvl 1 ,2-dimethylcyclohex-l-ene-4,5-dicarboxylate (2.26g, O.Olmol) in dry THF (40ml) over 30 in. The resultant suspension was stirred for another hour at 0 C, and then saturated sodium sulphate solution (5ml) was added dropwise. The solution was filtered and the filtrate evaporated to give an oil, which on purification gave the title compound in 36% yield. N.m.r: δH (90MHz, CDC1 ₃ ) 3.70 (m,4H), 3.55 (bs,2H), 2.02 (m,4H), 1.96 (m,2H), 1.64 ppm (s,6H).

B. Trans-4.5-bis(aminomethyl)-l -2-dimethylcyclohex-l-ene dihvdrochloride

The procedure of Example 1 above was repeated using ±ranι-4,5-bis(hydroxymethyl)-l ,2-d1methylcyclohex-l-ene as the starting diol. The title compound was obtained in 22% yield. N.m.r: δH (90MHz, D ₂ 0) 3.42 (m,4H), 2.05 (m,4H), 1.80 (m,2H), 1.66 ppm (s,6H).

Example 5: Synthesis of cis-4.5-bis(aminomethvP- 1.2-dimethylcvclohex-l-ene dihvdrochloride

A. Cis-1.2-dimethylcyc1ohexene-4.5-dicarboxylic anhydride Maleic anhydride (9.8g, 0.10 ol) and 2,3-dimethylbutadiene (9.84g, 0.12 mol) were mixed in toluene (50 ml) for 24h at 50°C. The solution was allowed to cool, the solvent removed in vacuo and the residue recrystallised from petroleum ether (40°C-60°C) to yield the title compound in 92% yield. N.m.r: δH (90MHz, CDC1 ₃ ) 2.60 (m,2H), 2.14 (m,4H), 1.74 (s,6H).

B. Cis-4.5-bis(aminomethyl)-l .2-dimethylcvclohex-l-ene dihvdrochloride

The procedures for the conversion from the anhydride into the title compound were as described in Example 3. Overall yield 6%. N.m.r: δH (90MHz, D ₂ 0) 3.37 (m,4H), 2.02 (m,4H) 1.76 (m,2H), 1.65 (s,6H).

Example 6: Synthesis of trans-4.5-bis(diethylaminomethyl ) -1 -2-dimethylcyclohex-l-enedihydrochloride

A. Trans-dimethyl 1.2-dimethylcvclohex-l-ene-4.5-dicarboxy1ate

2,3-dimethylbutadiene (9.84g, 0.12mol) and dimethyl fumarate

(14.4g, O.lmol) were stirred together in toluene (40ml) at 50 C for 24hr. The solvent was removed in vacuo and the solid residue recrystallised from diethyl ether, to give the title compound in 85% yield. N.m.r: δH (90MHz, CD ₃ 0D) 3.71 (s,6H), 2.55 (m,2H), 2.10 (m,4H), 1.74 ppm (s,6H).

B. Trans-1.2-d1methylcyclohex-l-ene-4.5-dicarboxyl1c acid

Trans-dimethyl 1 ,2-dimethylcyclohex-l-ene-4,5-dicarboxylate ⁽ 4.52g, 0.02mol) was refluxed with NaOH (4.0g, O.lmol) in methanol (40ml) for 4hr. The solution was allowed to cool and partitioned between aqueous NaOH (2M, 50ml) and ether (60ml). The aqueous layer was then acidified (1M HC1) and extracted with ethyl acetate (3x50ml). The solvent was removed in vacuo to give the title compound in 77% yield. N.m.r: δH (90 MHz CD3CI3) 2.58 (m,2H), 2.08 (m,4H), 1.70 ppm (s,6H)

C. Trans-N.N.N' .N'-tetraethyl-l -2-dimethylcyclohex-l-ene-4.5- dicarboxamide

To ir ni-1 ,2-dimethylcyclohexene-4,5-dicarboxylic acid (1.98g O.Olmol) heated to 80°C, was added thionyl chloride (2.38g, 0.02mol) over lhr. The mixture was kept at this temperature for a further two hours, then cooled and added dropwise to a diethyl ether solution (45ml) of excess diethylamine (14.6g, 0.2mol) cooled to ice bath temperature.

The solvent was removed to give the crude product as an oil which was not purified for use in the next stage.

D. Trans-N.N.N' .N'-tetraethyl-4.5-bis(aminomethyl)-l .2- dimethylcvclohex-1-ene dihvdrochloride - trans-4.5-b1s(diethylaminomethyl)-1.2-dimethylcyclohex-l-ene dihvdrochloride

The crude diamide (1.54g, O.OOδmol) in THF (25ml) was added to a suspension of lithium aluminium hydride (0.76g, 0.02mol ⁾ in THF (30ml) at 25°C over 30 min. The resultant suspension was stirred for a further two hours at this temperature, then saturated sodium sulphate solution (5ml) was added. The solution was filtered, and partitioned between diethyl ether (50ml) and hydrochloric acid (1M, 50ml). The aqueous layer was further extracted with dichloromethane (2x50ml), and the water removed in vacuo to leave a brown solid, which was recrystallised to give the title compound in 5% overall yield. N.m.r: δH (90 MHz, D ₂ 0) 3.30 (m,12H), 2.14 (m,4H), 1.70 ppm (m,20H)

Example 7: Synthesis of trans-N.N.N' .N'-tetramethyl -1.2-cvc1ohexanebis(methanamino)dihydrochloride = trans-1.2-b1s(dimethylaminomethyl)cyclohexane hydrochloride

This synthesis was carried out by adapting the procedure described in L.D. Quin it al., J. Org. Chem. 44, 3496-3500

(1989) used to prepare cis-1 ,2-bis(dimethylaminomethyl) cyclohexane.

A. Trans-N.N.N' .N'-tetramethyl-l .2-cvclohexanedicarboxamide

Synthesis was followed as Quin et aj_. to give a white-solid (20% yield). N.m.r: δH (200 MHz, CDC1 ₃ ) 1.37 (4H,m) 1.82 (4H,m), 2.65(2H,m), 2.89 (6H,s) and 3.10(6H,s).

B. Trans-N.N.N' .N'-tetramethyl-l .2-cyclohexanebis(methanamine)

Again the procedure was followed as in Quin ei ______ to give a yellow oil (17% yield). N.m.r: δH (90 MHz, CDCI3) 1.3 (4H,m) 1.8-2.3(6H,m), 3.0-3.2 (16H,m).

C. Trans-N.N.N' .N'-tetramethyl-l .2-cvclohexanebis(methanamino) dihvdrochloride *= trans-1.2-b1s(dimethylaminomethyl)cyclohexane dihvdrochloride

The free base was stirred with ethereal HC1 for 2 hours, to produce the required hydrochloride salt (90% yield). N.m.r: δH (90 MHz, D ₂ 0) 1.3 (4H,m), 1.8 (6H,m), 2.9-3.3 (16H,m).

Example 8: Synthesis of trans-1.2-bis(dimethylaminomethyl) cvclobutane dihvdrochloride

The procedure described above in Example 7 was repeated using trans-1.2-butanedicarboxylic acid, and a yellow solid was isolated as the hydrochloride salt in 10% overall yield. N.m.r: δH (90 MHz, CD ₃ 0D) 1.6-2.5 (6H,m), 2.7(16H,m).

Example 9: Effect of 1.2-bis(aminomethyl)-4.5-dimethylcyclohexa -1.4-diene ("BAD") on powdery mildew infection of barlev seedlings

The effect of the alicyclic polya ine analogue, BAD on powdery mildew infection of barley was studied. Seeds of barley (Hordeum vulαare L. cv Golden Promise) were sown in Fisons Levington compost in 36 cm seed trays. Plants were grown in a glasshouse under natural daylight supplemented for 16 hours daily by 400 W mercury vapour lamps. The maximum temperature was 24 ^β C during the day and fell to a minimum of 9 ^β C at night. Plants at growth stage 12 (second leaf unfolded, Zadok's scale) were used in experiments. Seedlings were sprayed to run-off with solutions of the compounds containing 0.01% Tween 20. In all cases solutions were adjusted to pH 7.0 prior to spraying (using either sodium hydroxide or HC1). Sprays were applied using a Shandon spray unit either before or after inoculation with powdery mildew. Plants were inoculated with mildew conidia by shaking infected stock plants over them. Intensity of infection was assessed 6, 8 and 10 days after inoculation by estimating the percentage leaf area infected using a standard area diagram. Barley powdery mildew normally sporulates 6-7 days after inoculation.

BAD, applied to barley seedlings as a 1 or 5 mM spray, pre- or post-inoculation, gave excellent control of powdery mildew. 1 mM BAD applied post-inoculation reduced mildew infection by 94%. This experiment was repeated three times with the same result.

The results of this experiment are shown in Tables 1 and 2 below.

Table 1: Effects of BAD on infection of barley seedlings with powdery mildew pre-inoculation.

Treatment % powdery mildew infection

days after inoculation 6 8 10

Control 5.3 ± 1.1 10.1 ± 1.8 13.8 ± 1.7

BAD, ImM 2.1 ± 0.2 2.8 ± 0.5 5.4 ± 0.7 BAD, 5mM 0.6 ± 0.1 1.6 ± 0.3 2.9 ± 0.4

Table 2: Effects of BAD on infection of barley seedlings with powdery mildew post-inoculation.

Treatment % powdery mildew infection

days after inoculation 6 8 10

Control 24.5 ± 1.6 43.2 ± 2.6 55.0 ± 3.1

BAD, ImM 2.5 ± 0.3 3.5 ± 0.4 3.9 ± 0.4 BAD, 5mM 0.6 ± 0.1 2.0 ± 0.2 2.8 ± 0.4

Example 10: Tests for systemic action of BAD - Root Drench Tests

Barley seedlings were grown and the experiments were carried out as described in Example 9. BAD applied as a pre-inoculation or a post-inoculation root drench resulted in some reduction in mildew infection. Mildew control was less pronounced for the pre-inoculation treatment, but was more substantial for post-inoculation treatments (72% reduction in mildew if applied 1 d after inoculation). Thus, BAD has xylem mobility and does give mildew control especially if applied as a post inoculation drench. The results are shown in Table 3 below:

Table 3: Effects of BAD, applied as a root drench, on mildew infection of barley seedlings. All treatments applied at ImM.

Treatment % mildew infection

days after inoculation

10

Pre- and post-inoculation root drench

Control 12.6 ± 1.2 25.0 ± 2.4 32.5 ± 1.8

BAD, 1 d pre 12.7 ± 1.3 20.5 ± 2.8 36.5 ± 2.3

BAD, 2 d pre 11.2 ± 1.8 17.4 ± 2.0 28.5 ± 4.1 BAD, 5 d pre 12.6 ± 1.2 23.8 ± 1.5 30.0 ± 3.1

Control 3.5 ± 0.4 6.4 ± 0.6 8.3 ± 0.8

BAD, 1 d post 2.0 ± 0.2 3.1 ± 0.4 4.9 ± 0.5

BAD, 2 d post 2.0 ± 0.2 1.9 ± 0.2 2.5 ± 0.3

BAD, 5 d post 2.2 ± 0.3 3.0 ± 0.4 5.5 ± 0.7

Example 11 : Effects of applying BAD to the lower leaves on powdery mildew infection on the upper leaves of barley seedlings

Barley seedlings were grown and the procedure carried out was as described in Example 9 hereinbefore.

The results are shown in Table 4 below.

Application of ImM BAD to the lower leaves 1, 2 or 5 days after inoculation of the upper leaves provided no control of powdery mildew.

Table 4

Effects of BAD, applied to lower leaves, on powdery mildew infection on the upper leaves of barley seedlings. BAD was applied as a post inoculation treatment at ImM, 1, 2 or 5 days after inoculation of the upper leaves with mildew.

Treatment % mildew infection

days after inoculation

10

Example 12: Effects of timing of the application of BAD on control of barley powdery mildew

Barley seedlings were grown and the procedure for the experiments was carried out as described in Example 9. However in this example, the experiments were conducted in order to investigate the effects of timing of the application of BAD to the seedlings at different times, before or after inoculation with the pathogen. The results are shown in Table 5 below.

Table 5: Effects of application at different times before or after inoculation, on powdery mildew infection of barley seedlings. All compounds applied at 1 mM. Assessments were made 6, 8 and 10 days after inoculation, but only the assessment on day 10 is shown.

% mildew infection

Control 31.0 % 2.5 5 d pre-inoculation BAD 20.0 % 0.8

2 d pre-inoculation BAD 20.0 % 4.3

1 d pre-inoculation BAD 22.8 % 2.4

1 d post-inoculation BAD 11.0 % 1.0

2 d post-inoculation BAD 6.8 % 1.2 5 d post-inoculation BAD 11.1 % 1.9

Although BAD reduced mildew infection irrespective of the timing of application, it was markedly less effective if applied prior to inoculation with the fungus. On the other hand, application after mildew inoculation gave very good control of mildew. It appears, therefore, that BAD has very little protective action against barley mildew, but does possess considerable curative action.

Example 13: Effects of BAD on infection of barley seedlings bv fungicide resistant strains of powdery mildew.

The effectiveness of 1 mM BAD on infection of barley seedlings by fungicide resistant strains of powdery mildew was examined. Again, Example 9 outlines the procedures followed. Three strains of barley powdery mildew were used, all supplied by the John Innes Centre for Plant Science Research, Cambridge Lab, Norwich, England: cc-146 resistant to ethirimol and triadimenol cc-138 resistant to triadimenol cc-139 resistant to fenpropidin and fenpropimorph BAD gave very good control of all of the strains of powdery mildew. In contrast, the fungicides "Mistral" (containing fenpropimorph) and "Bayfidan" (containing tridaimenol) gave poor control of strains cc-139 and cc-138 respectively.

Example 14: Comparison of the effects of BAD with commercial fungicides, on powdery mildew infection of barlev seedlings

BAD (ImM) was compared with the following active ingredients of commercial fungicides (all used at 0.1%): propiconazole, tridemorph, flutriafol, fenpropidin. Method Barley seedlings (Hordeum vulgare L. CV Golden Promise) were sown in Fisons Levington compost in 36 cm in seed trays. Plants were grown in a glasshouse under natural daylight supplemented for 16 h daily by 400 W mercury vapour lamps. The maximum temperature was 24 C during the day and fell to a minimum of 9°C at night. Plants at growth stage 12 (second leaf unfolded, Zadok's scale) were used in the experiments. Seedlings were sprayed to run-off with solutions of BAD at a concentration of ImM containing 0.01% "Tween" 20. In all cases solutions were adjusted to pH 7.0 prior to spraying (using either sodium hydroxide or HC1).

Solutions were sprayed onto seedlings until run off using a Shandon spray unit. Plants were inoculated with powdery mildew conidia by shaking infected stock plants over them. Intensity of infection was assessed 7 and 10 days after inoculation by estimating the percentage leaf area infected using a standard diagram. Sporulation usually occurred about 6-7 days after inoculation.

The results are shown in Table 6 below. Application of ImM

BAD as a post-inoculation spray to barley seedlings provided mildew control that was as good as that achieved with 0.1% propiconazole or tridemorph. Mildew control using BAD (78% control) was greater than that obtained using flutriafol (49%).

TABLE 6 Comparison of the effects of BAD with the active ingredients of commercial fungicides on powdery mildew infection of barley seedlings. Mildew was assessed 10 days after inoculation.

Treatment % powdery mildew infection

Control 4.5 BAD, 1 M 1.0 Propiconazole, 0.1 % 0.9

Tridemorph, 0.1 % 0.8

Flutriafol, 0.1 % 2.3

Penpropidin, 0.1 % 0.6

Example 15: Effect of BAD on infection of apple seedlings with the powdery mildew fungus Podosphaera leucotricha.

The effects of different concentrations of BAD, applied either as pre-inoculation or post-inoculation treatments, against the apple powdery mildew pathogen, Podosphaera leucotricha. were examined. Seeds of apple (Malus bitenfelder) were stratified by placing in a cold store for 14 weeks in trays of Fison's Levington compost. After 14 weeks the seeds were removed from cold storage and after 10 days those which had germinated were potted into individual 4 cm pots. After a further 12 days, the seedlings were inoculated by gently brushing spores of the apple powdery mildew fungus Podosphaera leucotricha on to the leaves. Three days after inoculation, the seedlings were sprayed to run off with a solution of BAD (1 or 5 mM ⁾ using a Shandon spray unit. Inhibitor solutions were prepared in 0.01% "Tween" 20 and the pH adjusted to 7.0 using sodium hydroxide. Intensity of infection was assessed 13, 15 and 17 days after inoculation by estimating the percentage leaf area infected.

The results are shown in Table 7 below. BAD at 1 and 5 mM, applied before or after inoculation, gave very good control of apple powdery mildew. This suggests that against apple mildew, BAD possesses powerful protective and curative action (cf. barley mildew in Example 12).

TABLE 7 Effects of different concentrations of BAD, applied before or after inoculation, on powdery mildew infection of apple seedlings. Only the final assessment, 17 days after inoculation, is shown.

Treatment Infection index

Control III BAD 1 mM pre-inoculation I BAD 5 mM pre-inoculation I BAD 1 mM post-inoculation I BAD 5 mM post-inoculation I

Assessment Key 0 = no infection

I = infection just visible II = infection area < 50% III = infection area > 50%

Example 16: Effect of BAD on infection of broad bean seedings with the chocolate spot fungus. Botrytis fabae.

Method Seeds of broad bean (Vicia faba cv Express Long Pod) were sown in Fison's Levington compost 1n 15 cm plastic pots. Plants were grown in a ventilated glasshouse under natural daylight supplemented to a 16 h photoperiod with 400 W mercury vapour lamps. The maximum daylight temperature was 24°C, falling to a minimum of 9°C at night.

Twenty day-old plants were sprayed to run-off with solutions of BAD (1 or 5 mM) before or after inoculation with spores of Botrvtis fabae. Solutions were prepared in 0.01% "Tween" 20, with the pH adjusted to 7.0 with sodium hydroxide. Control plants

were sprayed with "Tween" 20 (0.01%) only. After inoculation with a spore suspension, plants were loosely covered in plastic bags for 48 h to maintain the high relative humidity necessary for spore germination. Plants to be given a pre-inoculatory treatment were sprayed with the inhibitors and left to dry for 2 h before inoculation. For post-1noculatory treatments, plants were Inoculated and left for 2 days before application of the inhibitor. Intensity of infection was assessed 3, 5 and 7 days after inoculation by estimating the percentage leaf area infected using a standard area diagram.

The results are shown in Table 8 below. Pre- and post- inoculation sprays of BAD (1 and 5 M) gave moderate control of chocolate spot on broad beans. Thus, a pest inoculation spray of 1 mM BAD reduced chocolate spot injection by 42%.

TABLE 8 Effects of BAD on infection of broad bean by the chocolate spot fungus Botrytis fabae

Treatment % leaf area Infected

Pre-inoculation sprays

Control 34.1 1 mM BAD 23.7

5 mM BAD 20.0

Post-inoculation sprays

Control 22.4

1 mM BAD 20.0 5 mM BAD 28.7

Example 17: Effect of BAD on infection of broad beans with the rust fungus. Uromvces viciae-fabae.

Method These experiments were performed using the method described above in Example 16 for infection of beans with Botrvtis fabae. For the rust experiments, BAD was used at 1 and 5 M concentrations and assessment of intensity of infection was carried out 12, 15 and 18 days after inoculation. The results are shown in Table 9 below.

TABLE 9 Effects of a post-inoculation treatment with BAD on infection of faba beans by the rust fungus, Uromyces viciae-fabae.

Treatment % rust infection

days after inoculation 12 15 18

Control 19.1 ± 2.5 22.5 ± 3.2 26.6 ± 4.0

BAD, 1 mM 20.4 ± 2.5 24.4 ± 2.8 25.4 ± 3.3

BAD, 5 M 21.6 ± 3.9 22.0 ± 3.8 24.0 ± 4.5

Example 18: Effects of the allevel1c diamines of Examples 3-5 on powdery mildew infection of barley seedlings.

The method followed was as described in Example 9 hereinbefore. The results are shown in Table 10 below. Post-inoculation treatment with the Ex.4 compound at 1 mM provided substantial control of powdery mildew infection on barley seedlings.

TABLE 10 Effects of alicyclic diamines of Examples 3-5 on powdery mildew infection of barley seedlings. Mildew was assessed 10 days after inoculation.

Treatment % powdery mildew infection

Control 13.9 + 0.8

Cpd. of Ex.4, 1 mM 4.7 + 0.3

Control 16.0 + 2.0

Cpd. of Ex.5, 1 mM 12.6 + 1.4

Cpd. of Ex.3, 1 mM 16.5 + 1.1

Example 19: Effects of the compound of Ex. 4 on infection of potato leaf discs with the blight fungus Phytophthora infestans.

Potato leaf discs were floated on a solution of BAD at ImM and 5mM concentration in a petri dish. They were then inoculated with sporangia of the blight fungus Phytophthora Infestans and the covered petri dishes were then incubated for 6 days. The results are shown in Table 11 below. The compound of Example 4 at 1 and 5 M provided moderate control of blight infection of potato leaf discs.

TABLE 11 Effects of the compound of Example 4 on infection of potato leaf discs with Phytophthora infestans.

Treatment Infection index *

Control 5

Cpd. of Ex.4, 1 mM 3.5

Cpd. of Ex.4, 5 mM 2.5

Infection index 5 leaf disc covered with blight 0 no blight on leaf disc

Example 20: Effects of the compounds of Examples 1. 6. 7 and 8 on powdery mildew infection of barlev seedlings.

The experimental procedure followed was as described in Example 9 hereinbefore. The results are shown in Table 12 below. The compounds were applied as post-inoculation sprays and the results presented here are infection intensities at 6 days after inoculation. All compounds reduced mildew infection significantly. The smallest reduction in mildew was achieved using the compound of Example 6 (58%), at the low concentration specified, while the greatest reduction in mildew Infection was obtained using the compound of Example 8 (75%), at a higher concentration.

TABLE 12

Treatment % mildew Infection

Control 3.36 ± 0.24

Compound of Ex.7 (1.23 mM) 0.93 ± 0.11

Compound of Ex.8 (1 M) 0.85 ± 0.15

Compound of Ex.1 (1 M) 1.17 ± 0.13

Compound of Ex.6 (0.28 mM) 1.43 ± 0.12