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Title:
ALLERGEN DETECTION MARKER AND ALLERGEN DETECTION METHOD USING SAME
Document Type and Number:
WIPO Patent Application WO/2021/039147
Kind Code:
A1
Abstract:
The present invention provides a highly sensitive allergen measurement method. This method for detecting allergens in a sample comprises: treating the sample with a protease; and detecting, through an analysis using chromatographic separation, the presence or absence of an allergen marker in the sample on which the enzymatic treatment has been performed. The allergens include buckwheat or wheat.

Inventors:
AKIYAMA HIROSHI (JP)
KIKUCHI HIROYUKI (JP)
SEKI YUSUKE (JP)
ARIMOTO CHISATO (JP)
Application Number:
PCT/JP2020/026896
Publication Date:
March 04, 2021
Filing Date:
July 09, 2020
Export Citation:
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Assignee:
JAPAN AS REPRESENTED BY DIRECTOR GENERAL OF NAT INSTITUTE OF HEALTH SCIENCES (JP)
NISSHIN SEIFUN GROUP INC (JP)
International Classes:
C07K14/415; C12Q1/37; G01N27/62; G01N30/06; G01N30/72; G01N30/88; G01N33/10
Domestic Patent References:
WO2018016551A12018-01-25
WO2003066079A22003-08-14
WO2018016551A12018-01-25
Foreign References:
JP2014525588A2014-09-29
JP2006126083A2006-05-18
JP2014525588A2014-09-29
JP2013539039A2013-10-17
Other References:
JIRA, W. ET AL.: "A sensitive HPLC-MS/MS screening method for the simultaneous detection of barley, maize, oats, rice, rye and wheat proteins in meat products", FOOD CHEMISTRY, vol. 275, 2019, pages 214 - 223, XP085515079, DOI: 10.1016/j.foodchem.2018.09.041
MARTINEZ-ESTESO, M. J. ET AL.: "Defining the wheat gluten peptide fingerprint via a discovery and targeted proteomics approach", JOURNAL OF PROTEOMICS, vol. 147, 2016, pages 156 - 168, XP029698916, DOI: 10.1016/j.jprot.2016.03.015
YOSHIOKA, H. ET AL.: "Expression and epitope analysis of the major allergenic protein Fag e 1 from buckwheat", JOURNAL OF PLANT PHYSIOLOGY, vol. 161, 2004, pages 761 - 767, XP004955349, DOI: 10.1016/j.jplph.2004.01.010
See also references of EP 4023664A4
Attorney, Agent or Firm:
THE PATENT CORPORATE BODY ARUGA PATENT OFFICE (JP)
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