Title:
ALLULOSE 3-EPIMERASE MUTANT, ENGINEERED BACTERIUM EXPRESSING SAME, AND IMMOBILIZED ENZYME AND IMMOBILIZATION METHOD THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/244005
Kind Code:
A1
Abstract:
Provided are an allulose 3-epimerase mutant and a genetically engineered bacterium expressing the mutant. Further provided are an immobilized allulose 3-epimerase enzyme and an immobilization method thereof. A high-throughput screening method is used to obtain an allulose 3-epimerase mutant efficiently expressed in a fermentation process, which can catalyze efficient conversion of fructose to D-allulose, providing an efficient production path for key enzymes required in a D-allulose production process. Additionally, the allulose 3-epimerase is bonded to an immobilizing resin to prepare an immobilized allulose 3-epimerase enzyme. The immobilized enzyme can be applied to batch or continuous reactions to catalyze efficient conversion of fructose to D-allulose, improves the reuse rate and useful life of the enzyme, can significantly reduce the production costs of D-allulose, and can be widely applied.
Inventors:
ZHU YUEMING (CN)
CHEN PENG (CN)
SUN YUANXIA (CN)
ZENG YAN (CN)
YANG JIANGANG (CN)
MEN YAN (CN)
MA YANHE (CN)
CHEN PENG (CN)
SUN YUANXIA (CN)
ZENG YAN (CN)
YANG JIANGANG (CN)
MEN YAN (CN)
MA YANHE (CN)
Application Number:
PCT/CN2020/136574
Publication Date:
December 09, 2021
Filing Date:
December 15, 2020
Export Citation:
Assignee:
TIANJIN INST IND BIOTECHNOLOGY CAS (CN)
International Classes:
C12N9/90; A23L29/30; C12P19/24
Domestic Patent References:
| WO2018116266A1 | 2018-06-28 |
Foreign References:
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Other References:
ZHANGLIANG ZHU, DENGKE GAO, CHAO LI, YING CHEN, MENGLU ZHU, XIN LIU, MASARU TANOKURA, HUI-MIN QIN, FUPING LU: "Redesign of a novel d-allulose 3-epimerase from Staphylococcus aureus for thermostability and efficient biocatalytic production of d-allulose", MICROBIAL CELL FACTORIES, vol. 18, no. 1, 1 December 2019 (2019-12-01), XP055702883, DOI: 10.1186/s12934-019-1107-z
Attorney, Agent or Firm:
BEIJING ORIGINTELLIGENCE IP LAW FIRM (CN)
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