Title:
ANTI-MYL9 ANTIBODY
Document Type and Number:
WIPO Patent Application WO/2017/122666
Kind Code:
A1
Abstract:
[Problem] To provide: an anti-Myl9 antibody which can bind to Myl9 to inhibit the interaction between Myl9 and CD69 in a human body, or an Myl9-binding fragment of the antibody; and a pharmaceutical composition containing the antibody or the fragment.
[Solution] A mouse anti-human/mouse Myl9 monoclonal antibody which has binding affinity for Myl9 was produced, and the sequences for complementarity determining regions (CDRs) of the mouse anti-human/mouse Myl9 monoclonal antibody were identified. Thus, a humanized antibody is produced, in which heavy-chain variable regions and light-chain variable regions respectively contain the CDR sequences of the mouse anti-human/mouse Myl9 monoclonal antibody.
Inventors:
NAKAYAMA TOSHINORI (JP)
KIMURA MOTOKO (JP)
HAYASHIZAKI KOJI (JP)
HIRAYAMA TOSHIFUMI (JP)
KAKUTA JUNGO (JP)
SAKAMOTO YOSHIMASA (JP)
GEJIMA RYU (JP)
TOKITA DAISUKE (JP)
MURAMOTO KENZO (JP)
IMAI TOSHIO (JP)
KIMURA MOTOKO (JP)
HAYASHIZAKI KOJI (JP)
HIRAYAMA TOSHIFUMI (JP)
KAKUTA JUNGO (JP)
SAKAMOTO YOSHIMASA (JP)
GEJIMA RYU (JP)
TOKITA DAISUKE (JP)
MURAMOTO KENZO (JP)
IMAI TOSHIO (JP)
Application Number:
PCT/JP2017/000605
Publication Date:
July 20, 2017
Filing Date:
January 11, 2017
Export Citation:
Assignee:
NAT UNIV CORP CHIBA UNIV (JP)
EISAI R&D MAN CO LTD (JP)
EISAI R&D MAN CO LTD (JP)
International Classes:
C07K16/18; A61K39/395; A61P1/04; A61P11/00; A61P35/00; A61P37/08; C07K16/46; C12N15/09
Domestic Patent References:
| WO2014192915A1 | 2014-12-04 | |||
| WO2015190538A1 | 2015-12-17 | |||
| WO2010123012A1 | 2010-10-28 | |||
| WO2014205501A1 | 2014-12-31 | |||
| WO2008105058A1 | 2008-09-04 | |||
| WO2014192915A1 | 2014-12-04 |
Other References:
LUO XUE-GANG ET AL.: "Histone methyltransferase SMYD3 promotes MRTF-A-mediated transactivation of MYL9 and migration of MCF-7 breast cancer cells", CANCER LETTERS, vol. 344, 2014, pages 129 - 137, XP028826472
ISHIHARA S. ET AL.: "Irradiation-tolerant lung cancer cells acquire invasive ability dependent on dephosphorylation of the myosin regulatory light chain", FEBS LETTERS, vol. 587, 2013, pages 732 - 736, XP028998881
TESTI, R. ET AL., IMMUNOL. TODAY, vol. 15, 1994, pages 479 - 483
MURATA, K. ET AL.: "CD69-null mice protected from arthritis induced with anti-type II collagen antibodies", INT. IMMUNOL., vol. 15, 2003, pages 987 - 992
MIKI-HOSOKAWA, T. ET AL.: "CD69 controls the pathogenesis of allergic airway inflammation", J. IMMUNOL., vol. 183, 2009, pages 8203 - 8215, XP055219366, DOI: doi:10.4049/jimmunol.0900646
"Genbank", Database accession no. NP 006088.2
"Genbank", Database accession no. NP_742116.1
KABAT ET AL.: "Sequences of Proteins of Immunological Interest", 1991, NATIONAL INSTITUTES OF HEALTH
AL-LAZIKANI ET AL., J. MOLEC. BIOL., vol. 273, 1997, pages 927 - 948
JONES ET AL., NATURE, vol. 321, 1986, pages 522 - 525
RIECHMANN ET AL., NATURE, vol. 332, 1988, pages 323 - 327
VERHOEYEN ET AL., SCIENCE, vol. 239, 1988, pages 1534 - 1536
KONTERMANN; DUBEL, ANTIBODY ENGINEERING, SPRINGER LAB MANUAL, 2001
TSURUSHITA ET AL., METHODS, vol. 36, 2005, pages 69 - 83
CARTER ET AL., PROC. NATL. ACAD. SET USA, vol. 89, 1992, pages 4285 - 4289
PRESTA ET AL., J. IMMUNOL., vol. 151, 1993, pages 2623 - 2632
"Genbank", Database accession no. NP-006088.2
"Genbank", Database accession no. NP_034989.1
"Genbank", Database accession no. NP_000249.1
POWRIE ET AL., INT. IMMUNOL., vol. 5, 1993, pages 1461 - 1471
COOPER ET AL., LAB. INVEST., vol. 69, 1993, pages 238 - 249
"Genbank", Database accession no. NP_080340.2
"Genbank", Database accession no. NP_075891.1
"Genbank", Database accession no. NP_001289976.1
"Genbank", Database accession no. NP 001138416.1
See also references of EP 3404040A4
ISHIHARA S. ET AL.: "Irradiation-tolerant lung cancer cells acquire invasive ability dependent on dephosphorylation of the myosin regulatory light chain", FEBS LETTERS, vol. 587, 2013, pages 732 - 736, XP028998881
TESTI, R. ET AL., IMMUNOL. TODAY, vol. 15, 1994, pages 479 - 483
MURATA, K. ET AL.: "CD69-null mice protected from arthritis induced with anti-type II collagen antibodies", INT. IMMUNOL., vol. 15, 2003, pages 987 - 992
MIKI-HOSOKAWA, T. ET AL.: "CD69 controls the pathogenesis of allergic airway inflammation", J. IMMUNOL., vol. 183, 2009, pages 8203 - 8215, XP055219366, DOI: doi:10.4049/jimmunol.0900646
"Genbank", Database accession no. NP 006088.2
"Genbank", Database accession no. NP_742116.1
KABAT ET AL.: "Sequences of Proteins of Immunological Interest", 1991, NATIONAL INSTITUTES OF HEALTH
AL-LAZIKANI ET AL., J. MOLEC. BIOL., vol. 273, 1997, pages 927 - 948
JONES ET AL., NATURE, vol. 321, 1986, pages 522 - 525
RIECHMANN ET AL., NATURE, vol. 332, 1988, pages 323 - 327
VERHOEYEN ET AL., SCIENCE, vol. 239, 1988, pages 1534 - 1536
KONTERMANN; DUBEL, ANTIBODY ENGINEERING, SPRINGER LAB MANUAL, 2001
TSURUSHITA ET AL., METHODS, vol. 36, 2005, pages 69 - 83
CARTER ET AL., PROC. NATL. ACAD. SET USA, vol. 89, 1992, pages 4285 - 4289
PRESTA ET AL., J. IMMUNOL., vol. 151, 1993, pages 2623 - 2632
"Genbank", Database accession no. NP-006088.2
"Genbank", Database accession no. NP_034989.1
"Genbank", Database accession no. NP_000249.1
POWRIE ET AL., INT. IMMUNOL., vol. 5, 1993, pages 1461 - 1471
COOPER ET AL., LAB. INVEST., vol. 69, 1993, pages 238 - 249
"Genbank", Database accession no. NP_080340.2
"Genbank", Database accession no. NP_075891.1
"Genbank", Database accession no. NP_001289976.1
"Genbank", Database accession no. NP 001138416.1
See also references of EP 3404040A4
Attorney, Agent or Firm:
NANJO, Masahiro et al. (JP)
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