Title:
AN ANTI SERUM ALBUMIN FAB-EFFECTOR MOIETY FUSION CONSTRUCT, AND THE PREPARING METHOD THEREOF
Document Type and Number:
WIPO Patent Application WO/2015/030539
Kind Code:
A1
Abstract:
The present invention relates to antigen-binding fragment(Fab) and a Fab-effector fusion protein or (poly)peptide comprising thereof. The Fab of the present invention specifically binds to serum albumin and thereby has extended in vivo half-life. The Fab of the present invention is characterized by not having cysteine residues that are responsible for the interchain disulfide bond in CH1 domain and CκL domain as well. The Fab-effector fusion protein or (poly)peptide of the present invention can be produced in periplasm of E. coli with high yield, and has increased in vivo half-life. Further, the present invention provides E. coli strain which produces various kinds of Fab-effector fusion proteins or (poly)peptides, and a pharmaceutical composition comprising the fab-effector fusion proteins or (poly)peptides.
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Inventors:
CHA SANGHOON (KR)
Application Number:
PCT/KR2014/008106
Publication Date:
March 05, 2015
Filing Date:
August 29, 2014
Export Citation:
Assignee:
APRILBIO CO LTD (KR)
International Classes:
C07K16/18; A61K39/395; C07K16/46
Domestic Patent References:
| WO2010063818A2 | 2010-06-10 | |||
| WO2005118642A2 | 2005-12-15 |
Foreign References:
| KR20120133403A | 2012-12-10 | |||
| KR20070073886A | 2007-07-10 | |||
| KR20070041781A | 2007-04-19 | |||
| KR20110008086A | 2011-01-25 | |||
| US20090111745A1 | 2009-04-30 |
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SOARES ET AL., PROTEIN ENGINEERING, vol. 16, 2003, pages 1131 - 1138
WALKER ET AL., PROTEIN ENG DES SEL., vol. 23, 2010, pages 271 - 278
ENTWISTLE ET AL., CANCER BIOTHER RADIOPHANN, vol. 27, 2012, pages 582 - 92
ILACK ET AL., TOXICOLOGY, vol. 185, 2003, pages 161 - 174
CHOE ET AL., CANCER RES., vol. 54, 1994, pages 3460 - 3467
KREITMAN ET AL., INT. J. CANCER, vol. 57, 1994, pages 856 - 864
LU ET AL., J IMMUNOLOG METH., vol. 267, 2002, pages 213 - 226
HOLZER ET AL., CYTOKINE, vol. 8, 1996, pages 214 - 221
SJOGAARD ET AL., INT J ONCOL., vol. 15, 1999, pages 873 - 882
HUMPHREYS ET AL., J. IMMUNOL. METHODS, vol. 209, pages 193 - 202
CARTER ET AL., BIOTECHNOLOGY (N Y), vol. 10, pages 163167
VENTURI ET AL., J MOL BIOL., vol. 315, pages 1 - 8
DONZEAU ET AL., METHODS MOL BIOL., vol. 378, pages 14 - 31
CLARK ET AL., J. BIOL. CHEM., vol. 271, 1996, pages 21969 - 21977
PRADHANANGA ET AL., J MOL ENDOCRINOL., vol. 29, 2002, pages 1114
SONDERGAARD ET AL., J CLIN ENDOCRINOL METABOL., vol. 96, 2011, pages 681 - 688
PALANKI ET AL., BIOORG. MED. CHEM. LETT., vol. 23, 2013, pages 402 - 406
OSBORN ET AL., EUR J PHARMACOL., vol. 456, 2002, pages 149 - 158
ANDERSON ET AL., J BIOL CHEM., vol. 286, 2011, pages 5234 - 5241
SLEEP ET AL., BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1830, 2013, pages 5526 - 5534
SCHLAPSCHY ET AL., PROTEIN ENG DES SEL., vol. 26, 2013, pages 489 - 501
SCHELLENBERGER ET AL., NAT BIOTECH., vol. 27, 2007, pages 1186 - 1190
CLELAND ET AL., J PHARM SCI., vol. 101, 2012, pages 27442754 - 2754
YUEN ET AL., J CLIN ENDOCRINOL METAB., vol. 98, 2013, pages 2595 - 2603
THOMAS JMERIMEE, DIABETES, vol. 22, 1973, pages 813 - 819
JOHN POWELL-JACKSON ET AL., LANCET, vol. 2, 1985, pages 244 - 246
BRUCE R.BLAZAR, THE JOURNAL OF IMMUNOLOGY, vol. 171, 2003, pages 1272 - 1277
A IMAGAWA ET AL., THE JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, vol. 80, 1995, pages 922 - 926
ANKE FRANZKE ET AL., BLOOD, vol. 102, 2003, pages 734 - 739
VAN THIEL DH ET AL., HEPATO-GASTROENTEROLOGY, vol. 42, 1995, pages 907 - 912
DENNIS ET AL., BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1830, 2002, pages 5526 - 5534
SCHLAPSCHY ET AL.: "Escherichia coli", PROTEIN ENG DES SEL., vol. 19, 2006, pages 385 - 390, XP002453523, DOI: 10.1093/protein/gzl018
KONTERMANN, CURR OP BIOTECHNOL., vol. 22, 2011, pages 868 - 876
UJHELYIROBERT, CLIN PHARMACOKINET., vol. 28, 1995, pages 483493
HOLT ET AL., PROTEIN ENG DES SEL., vol. 21, 2008, pages 283 - 288
DUDGEON ET AL., PROTEIN ENG DES SEL., vol. 22, 2009, pages 217 - 220
KIRSH ET AL., J IMMUNOL METHODS, vol. 301, 2005, pages 173 - 185
ROTHLISBERGER ET AL., J. MOL. BIOL., vol. 347, 2005, pages 773 - 789
HUMPHREYS ET AL., PROTEIN ENG DES SEL., vol. 20, 2007, pages 227 - 234
SOCKOLOSKYSZOKA, PROTEIN EXP PURIF., vol. 87, 2013, pages 129 - 135
SOARES ET AL., PROTEIN ENGINEERING, vol. 16, 2003, pages 1131 - 1138
WALKER ET AL., PROTEIN ENG DES SEL., vol. 23, 2010, pages 271 - 278
Attorney, Agent or Firm:
NAM & NAM WORLD PATENT & LAW FIRM (Seosomun-roJung-gu, Seoul 100-813, KR)
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