Title:
ANTIBODIES TO THE ED-B DOMAIN OF FIBRONECTIN, CONJUGATES CONTAINING THEM AND USE THEREFOR FOR DIAGNOSIS AND THERAPY OF TUMORS AND DISEASES ASSOCIATED WITH ANGIOGENESIS
Document Type and Number:
WIPO Patent Application WO1999058570
Kind Code:
A3
Abstract:
The present invention relates to antibodies with sub-nanomolar affinity specific for a characteristic epitope of the ED-B domain of fibronectin, a marker of angiogenesis. Furthermore, it relates to the use of radiolabeled high affinity anti ED-B antibodies for detecting new-forming blood vessels in vivo and a diagnostic kit comprising said antibody. Furthermore, it relates to conjugates comprising said antibodies and suitable photoactive molecules (e.g. a judiciously chosen photosensitizer), and their use for the selective light-mediated occlusion of new blood vesels.
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Inventors:
NERI DARIO
TARLI LORENZO
VITI FRANCESCA
BIRCHLER MANFRED
TARLI LORENZO
VITI FRANCESCA
BIRCHLER MANFRED
Application Number:
PCT/EP1999/003210
Publication Date:
March 16, 2000
Filing Date:
May 11, 1999
Export Citation:
Assignee:
EIDGENOESS TECH HOCHSCHULE (CH)
International Classes:
A61K39/395; A61K41/00; A61K47/48; C12N15/09; A61K49/00; A61K51/10; A61P35/00; C07K16/00; C07K16/18; A61K38/00; (IPC1-7): C07K16/18; A61K47/48; C12N15/13; A61K49/00
Domestic Patent References:
| WO1997045544A1 | 1997-12-04 |
Other References:
NERI D ET AL: "Targeting by affinity-matured recombinant antibody fragments of an angiogenesis associated fibronectin isoform.", NATURE BIOTECHNOLOGY, (1997 NOV) 15 (12) 1271-5., XP002124779
NERI, DARIO (1) ET AL: "Affinity reagents against tumour-associated extracellular molecules and newforming vessels.", ADVANCED DRUG DELIVERY REVIEWS, (APRIL 6, 1998) VOL. 31, NO. 1-2, PP. 43-52., XP002124780
PINI A ET AL: "Design and use of a phage display library. Human antibodies with subnanomolar affinity against a marker of angiogenesis eluted from a two-dimensional gel.", JOURNAL OF BIOLOGICAL CHEMISTRY, (1998 AUG 21) 273 (34) 21769-76., XP002124781
VITI F ET AL: "Increased binding affinity and valence of recombinant antibody fragments lead to improved targeting of tumoral angiogenesis.", CANCER RESEARCH, (1999 JAN 15) 59 (2) 347-52., XP002124782
FATTORUSSO R ET AL: "NMR structure of the human oncofoetal fibronectin ED-B domain, a specific marker for angiogenesis.", STRUCTURE, (1999 APR 15) 7 (4) 381-90., XP002124783
TARLI L ET AL: "A high-affinity human antibody that targets tumoral blood vessels.", BLOOD, (1999 JUL 1) 94 (1) 192-8., XP002124784
NERI, DARIO (1) ET AL: "Affinity reagents against tumour-associated extracellular molecules and newforming vessels.", ADVANCED DRUG DELIVERY REVIEWS, (APRIL 6, 1998) VOL. 31, NO. 1-2, PP. 43-52., XP002124780
PINI A ET AL: "Design and use of a phage display library. Human antibodies with subnanomolar affinity against a marker of angiogenesis eluted from a two-dimensional gel.", JOURNAL OF BIOLOGICAL CHEMISTRY, (1998 AUG 21) 273 (34) 21769-76., XP002124781
VITI F ET AL: "Increased binding affinity and valence of recombinant antibody fragments lead to improved targeting of tumoral angiogenesis.", CANCER RESEARCH, (1999 JAN 15) 59 (2) 347-52., XP002124782
FATTORUSSO R ET AL: "NMR structure of the human oncofoetal fibronectin ED-B domain, a specific marker for angiogenesis.", STRUCTURE, (1999 APR 15) 7 (4) 381-90., XP002124783
TARLI L ET AL: "A high-affinity human antibody that targets tumoral blood vessels.", BLOOD, (1999 JUL 1) 94 (1) 192-8., XP002124784
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