Title:
ANTIBODY RECOGNIZING EXTRACELLULAR REGION OF ALK2/ACVR1
Document Type and Number:
WIPO Patent Application WO/2021/020282
Kind Code:
A1
Abstract:
This application provides: an antibody which is usable not only as a therapeutic agent but also as an immunological and immunohistological analysis tool in immunostaining, western blotting, etc. and which specifically recognizes an extracellular region polypeptide of human, mouse and rat ALK2 and suppresses BMP signaling, or an antigen-binding fragment of the antibody; an antibody which has the property of specifically binding to the extracellular region of ALK2 to form an ALK2 homodimer and the property of inhibiting the formation of an ALK2-Type II receptor heterodimer and which inhibits the activation of ALK2 kinase or suppresses BMP signaling, or an antigen-binding fragment of the antibody; and a method for inhibiting the activation of ALK2 kinase or a method for suppressing BMP signaling with the use of the antibody or an antigen-binding fragment of the antibody.
Inventors:
KATAGIRI TAKENOBU (JP)
TSUKAMOTO SHO (JP)
KURATANI MAI (JP)
TSUKAMOTO SHO (JP)
KURATANI MAI (JP)
Application Number:
PCT/JP2020/028465
Publication Date:
February 04, 2021
Filing Date:
July 22, 2020
Export Citation:
Assignee:
UNIV SAITAMA MEDICAL (JP)
DAIICHI SANKYO CO LTD (JP)
DAIICHI SANKYO CO LTD (JP)
International Classes:
A61K39/395; A61P19/08; A61P25/00; A61P35/00; C07K16/28; C12N15/13; C12N15/63; G01N33/48; G01N33/53
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| WO2016121908A1 | 2016-08-04 | |||
| WO2009091048A1 | 2009-07-23 | |||
| WO2011027808A1 | 2011-03-10 | |||
| WO2012133572A1 | 2012-10-04 | |||
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KATAGIRI, TAKENOBU: "O-136 Identification of ALK2 extracellular region amino acid residues important for binding and inhibitory activity of the anti-ALK2 blocking antibodies", PROGRAMS AND ABSTRACTS OF THE ANNUAL MEETING OF THE JAPANESE SOCIETY FOR BONE AND MINERAL RESEARCH, vol. 37, 30 November 2018 (2018-11-30), pages 216, XP009534557
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T. KATAGIRI, J. ORAL BIOSCI., vol. 54, 2012, pages 119 - 123
T. KATAGIRIS. TSUKAMOTO, BIOL. CHEM., vol. 394, 2013, pages 703 - 714
E.M. SHORE ET AL., NAT. GENET., vol. 38, 2006, pages 525 - 527
T. KATAGIRI, J. ORAL BIOSCIENCES, vol. 54, 2012, pages 119 - 123
"GenBank", Database accession no. NM-001260761
SAIKI, R.K. ET AL., SCIENCE, vol. 239, 1988, pages 487 - 49
KOHLERMILSTEIN, NATURE, vol. 256, 1975, pages 495 - 497
PROC. NATL. ACAD. SCI. U.S.A., vol. 81, 1984, pages 6851 - 6855
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TOMIZUKA, K. ET AL., NATURE GENETICS, vol. 16, 1997, pages 133 - 143
KUROIWA, Y. ET AL., NUC. ACIDS RES., vol. 26, 1998, pages 3447 - 3448
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TOMIZUKA, K. ET AL., PROC. NATL. ACAD. SCI. USA, vol. 97, 2000, pages 722 - 727
WORMSTONE, I.M. ET AL., INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, vol. 43, no. 7, 2002, pages 2301 - 2308
CARMEN, S ET AL., BRIEFINGS IN FUNCTIONAL GENOMICS AND PROTEOMICS, vol. 1, no. 2, 2002, pages 189 - 203
SIRIWARDENA, D ET AL., OPHTHALMOLOGY, vol. 109, no. 3, 2002, pages 427 - 431
NATURE BIOTECHNOLOGY, vol. 23, no. 9, 2005, pages 1137 - 1146
ANNU. REV. IMMUNOL, vol. 12, 1994, pages 433 - 455
ACS CHEM. BIOL., vol. 7, 2012, pages 1848 - 1857
ACS CHEM. BIOL., vol. 11, 2016, pages 400 - 408
LORI BURTON ET AL., PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY, vol. 12, 2007, pages 265 - 273
SHIRRMANN, T. ET AL., MABS, vol. 2, no. 1, 2010, pages 1 - 4
MUYLDEMANS S. ET AL., PROTEIN ENG, vol. 7, no. 9, 1994, pages 1129 - 35
HAMERS-CASTERMAN C. ET AL., NATURE, vol. 363, no. 6428, 1993, pages 446 - 8
GLUZMAN, Y., CELL, vol. 23, 1981, pages 175 - 182
URLAUB, G.CHASIN, L.A., PROC. NATL. ACAD. SCI. U.S.A., vol. 77, 1980, pages 4126 - 4220
MOLECULAR IMMUNOLOGY, vol. 30, no. 1, 1993, pages 105 - 108
JOURNAL OF CHROMATOGRAPHY A, vol. 705, 1995, pages 129 - 134
ANALYTICAL BIOCHEMISTRY, vol. 360, 2007, pages 75 - 83
ALTSCHUL, STEPHEN F.THOMAS L. MADDENALEJANDRO A. SCHAFFERJINGHUI ZHANGZHENG ZHANGWEBB MILLERDAVID J. LIPMAN: "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", NUCLEIC ACIDS RES., vol. 25, 1997, pages 3389 - 3402, XP002905950, DOI: 10.1093/nar/25.17.3389
G.T. HERMANSON: "Strategies for Protein Purification and Characterization: A Laboratory Course Manual", 1996, COLD SPRING HARBOR LABORATORY PRESS
"Antibodies: A Laboratory Manual", 1988, COLD SPRING HARBOR LABORATORY
M.C. GARNET: "Targeted drug conjugates: principles and progress", ADVANCED DRUG DELIVERY REVIEWS, vol. 53, 2001, pages 171 - 216, XP002261805, DOI: 10.1016/S0169-409X(01)00227-7
D. PUTNAMJ KOPECEK: "Polymer Conjugates with Anticancer Activity", ADVANCES IN POLYMER SCIENCE, vol. 122, 1995, pages 55 - 123
X. YU ET AL., J NANOMATER, 2016
J. WANG ET AL., DELIVERY, vol. 25, no. 1, pages 1319 - 1327
D.D. LASIC: "Liposomes: From Physics to Applications", 1993, ELSEVIER SCIENCE PUBLISHERS B.V.
GENES CELLS, vol. 7, 2002, pages 949
BIOCHEM BIOPHYS RES COMMUN, vol. 407, 2011, pages 213
J BIOL CHEM, vol. 285, 2010, pages 15577
SCI REP, vol. 4, 2014, pages 7596
See also references of EP 4008350A4
KATAGIRI, TAKENOBU: "O-136 Identification of ALK2 extracellular region amino acid residues important for binding and inhibitory activity of the anti-ALK2 blocking antibodies", PROGRAMS AND ABSTRACTS OF THE ANNUAL MEETING OF THE JAPANESE SOCIETY FOR BONE AND MINERAL RESEARCH, vol. 37, 30 November 2018 (2018-11-30), pages 216, XP009534557
T. KATAGIRI, J. ORAL BIOSCI., vol. 52, 2010, pages 33 - 41
T. KATAGIRI, J. ORAL BIOSCI., vol. 54, 2012, pages 119 - 123
T. KATAGIRIS. TSUKAMOTO, BIOL. CHEM., vol. 394, 2013, pages 703 - 714
E.M. SHORE ET AL., NAT. GENET., vol. 38, 2006, pages 525 - 527
T. KATAGIRI, J. ORAL BIOSCIENCES, vol. 54, 2012, pages 119 - 123
"GenBank", Database accession no. NM-001260761
SAIKI, R.K. ET AL., SCIENCE, vol. 239, 1988, pages 487 - 49
KOHLERMILSTEIN, NATURE, vol. 256, 1975, pages 495 - 497
PROC. NATL. ACAD. SCI. U.S.A., vol. 81, 1984, pages 6851 - 6855
NATURE, vol. 321, 1986, pages 522 - 525
TOMIZUKA, K. ET AL., NATURE GENETICS, vol. 16, 1997, pages 133 - 143
KUROIWA, Y. ET AL., NUC. ACIDS RES., vol. 26, 1998, pages 3447 - 3448
LEE, H-S ET AL., MOLECULAR IMMUNOLOGY, vol. 36, 1999, pages 61 - 71
TOMIZUKA, K. ET AL., PROC. NATL. ACAD. SCI. USA, vol. 97, 2000, pages 722 - 727
WORMSTONE, I.M. ET AL., INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, vol. 43, no. 7, 2002, pages 2301 - 2308
CARMEN, S ET AL., BRIEFINGS IN FUNCTIONAL GENOMICS AND PROTEOMICS, vol. 1, no. 2, 2002, pages 189 - 203
SIRIWARDENA, D ET AL., OPHTHALMOLOGY, vol. 109, no. 3, 2002, pages 427 - 431
NATURE BIOTECHNOLOGY, vol. 23, no. 9, 2005, pages 1137 - 1146
ANNU. REV. IMMUNOL, vol. 12, 1994, pages 433 - 455
ACS CHEM. BIOL., vol. 7, 2012, pages 1848 - 1857
ACS CHEM. BIOL., vol. 11, 2016, pages 400 - 408
LORI BURTON ET AL., PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY, vol. 12, 2007, pages 265 - 273
SHIRRMANN, T. ET AL., MABS, vol. 2, no. 1, 2010, pages 1 - 4
MUYLDEMANS S. ET AL., PROTEIN ENG, vol. 7, no. 9, 1994, pages 1129 - 35
HAMERS-CASTERMAN C. ET AL., NATURE, vol. 363, no. 6428, 1993, pages 446 - 8
GLUZMAN, Y., CELL, vol. 23, 1981, pages 175 - 182
URLAUB, G.CHASIN, L.A., PROC. NATL. ACAD. SCI. U.S.A., vol. 77, 1980, pages 4126 - 4220
MOLECULAR IMMUNOLOGY, vol. 30, no. 1, 1993, pages 105 - 108
JOURNAL OF CHROMATOGRAPHY A, vol. 705, 1995, pages 129 - 134
ANALYTICAL BIOCHEMISTRY, vol. 360, 2007, pages 75 - 83
ALTSCHUL, STEPHEN F.THOMAS L. MADDENALEJANDRO A. SCHAFFERJINGHUI ZHANGZHENG ZHANGWEBB MILLERDAVID J. LIPMAN: "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", NUCLEIC ACIDS RES., vol. 25, 1997, pages 3389 - 3402, XP002905950, DOI: 10.1093/nar/25.17.3389
G.T. HERMANSON: "Strategies for Protein Purification and Characterization: A Laboratory Course Manual", 1996, COLD SPRING HARBOR LABORATORY PRESS
"Antibodies: A Laboratory Manual", 1988, COLD SPRING HARBOR LABORATORY
M.C. GARNET: "Targeted drug conjugates: principles and progress", ADVANCED DRUG DELIVERY REVIEWS, vol. 53, 2001, pages 171 - 216, XP002261805, DOI: 10.1016/S0169-409X(01)00227-7
D. PUTNAMJ KOPECEK: "Polymer Conjugates with Anticancer Activity", ADVANCES IN POLYMER SCIENCE, vol. 122, 1995, pages 55 - 123
X. YU ET AL., J NANOMATER, 2016
J. WANG ET AL., DELIVERY, vol. 25, no. 1, pages 1319 - 1327
D.D. LASIC: "Liposomes: From Physics to Applications", 1993, ELSEVIER SCIENCE PUBLISHERS B.V.
GENES CELLS, vol. 7, 2002, pages 949
BIOCHEM BIOPHYS RES COMMUN, vol. 407, 2011, pages 213
J BIOL CHEM, vol. 285, 2010, pages 15577
SCI REP, vol. 4, 2014, pages 7596
See also references of EP 4008350A4
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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