Title:
APPLICATION OF HETEROCYCLIC COMPOUND CONTAINING AT LEAST TWO SULFUR ATOMS IN PREPARING NANO-VACCINE AND PREPARED NANO-VACCINE
Document Type and Number:
WIPO Patent Application WO/2021/212858
Kind Code:
A1
Abstract:
The present invention pertains to the technical field of immunotherapy or disease prevention and treatment with vaccines, in particular to a heterocyclic compound containing two or more sulfur atoms and an application thereof in preparing a nano-vaccine. Provided is the application of the heterocyclic compound containing at least two sulfur atoms and capable of being covalently or non-covalently linked to a polypeptide in preparing the nano-vaccine. A nanoparticle prepared by self-assembly of the compound and an antigen can enter the dendritic cytoplasm in a membrane-crossing manner, thereby improving the uptake efficiency of the antigen and an immune adjuvant. In the process of entering a cell, the nano-vaccine can effectively avoid or reduce biodegradation of the antigen or nucleic acid adjuvant caused by enzymes in lysosomes, and therefore the nano-vaccine can efficiently activate the dendritic cells and improve the cross-presentation of the antigen, thereby effectively activating CD8+T cells and promoting T cell proliferation. Therefore, the nano-vaccine can prevent tumor cell proliferation and virus infection by efficient immune activation and immune regulation.
Inventors:
YANG HUANGHAO (CN)
LI JUAN (CN)
ZHANG DA (CN)
LIU XIAOLONG (CN)
LI JUAN (CN)
ZHANG DA (CN)
LIU XIAOLONG (CN)
Application Number:
PCT/CN2020/134162
Publication Date:
October 28, 2021
Filing Date:
December 07, 2020
Export Citation:
Assignee:
UNIV FUZHOU (CN)
International Classes:
A61K39/00; A61K9/14; A61K31/7088; A61K47/20; A61K47/54; A61P31/04; A61P31/12; A61P35/00
Foreign References:
| CN109758588A | 2019-05-17 | |||
| IN2148DEN2004A | 2007-08-17 | |||
| CN202010330996A | 2020-04-24 |
Other References:
ZHOU JIE, SHAO ZHENTAO, LIU JIA, DUAN QIAO, WANG XIANG, LI JUAN, YANG HUANGHAO: "From Endocytosis to Nonendocytosis: The Emerging Era of Gene Delivery", ACS APPLIED BIO MATERIALS, vol. 3, no. 5, 18 May 2020 (2020-05-18), US , pages 2686 - 2701, XP055860751, ISSN: 2576-6422, DOI: 10.1021/acsabm.9b01131
ZHANG DA, LIN ZIGUO, WU MING, CAI ZHIXIONG, ZHENG YOUSHI, HE LEI, LI ZHENLI, ZHOU JIE, SUN LIQIN, CHEN GENG, ZENG YONGYI, LI JUAN,: "Cytosolic Delivery of Thiolated Neoantigen Nano‐Vaccine Combined with Immune Checkpoint Blockade to Boost Anti‐Cancer T Cell Immunity", ADVANCED SCIENCE, vol. 8, no. 6, 1 March 2021 (2021-03-01), pages 2003504, XP055860755, ISSN: 2198-3844, DOI: 10.1002/advs.202003504
BARTOLAMI ELINE, BASAGIANNIS DIMITRIS, ZONG LILI, MARTINENT RÉMI, OKAMOTO YASUNORI, LAURENT QUENTIN, WARD THOMAS R., GONZALEZ‐GAIT: "Diselenolane‐Mediated Cellular Uptake: Efficient Cytosolic Delivery of Probes, Peptides, Proteins, Artificial Metalloenzymes and Protein‐Coated Quantum Dots", CHEMISTRY - A EUROPEAN JOURNAL, vol. 25, no. 16, 15 March 2019 (2019-03-15), pages 4047 - 4051, XP055860760, ISSN: 0947-6539, DOI: 10.1002/chem.201805900
GASPARINI GIULIO, BANG EUN-KYOUNG, MOLINARD GUILLAUME, TULUMELLO DAVID V., WARD SANDRA, KELLEY SHANA O., ROUX AURELIEN, SAKAI NAOM: "Cellular Uptake of Substrate-Initiated Cell-Penetrating Poly(disulfide)s", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 136, no. 16, 23 April 2014 (2014-04-23), pages 6069 - 6074, XP055860766, ISSN: 0002-7863, DOI: 10.1021/ja501581b
See also references of EP 4140500A4
ZHANG DA, LIN ZIGUO, WU MING, CAI ZHIXIONG, ZHENG YOUSHI, HE LEI, LI ZHENLI, ZHOU JIE, SUN LIQIN, CHEN GENG, ZENG YONGYI, LI JUAN,: "Cytosolic Delivery of Thiolated Neoantigen Nano‐Vaccine Combined with Immune Checkpoint Blockade to Boost Anti‐Cancer T Cell Immunity", ADVANCED SCIENCE, vol. 8, no. 6, 1 March 2021 (2021-03-01), pages 2003504, XP055860755, ISSN: 2198-3844, DOI: 10.1002/advs.202003504
BARTOLAMI ELINE, BASAGIANNIS DIMITRIS, ZONG LILI, MARTINENT RÉMI, OKAMOTO YASUNORI, LAURENT QUENTIN, WARD THOMAS R., GONZALEZ‐GAIT: "Diselenolane‐Mediated Cellular Uptake: Efficient Cytosolic Delivery of Probes, Peptides, Proteins, Artificial Metalloenzymes and Protein‐Coated Quantum Dots", CHEMISTRY - A EUROPEAN JOURNAL, vol. 25, no. 16, 15 March 2019 (2019-03-15), pages 4047 - 4051, XP055860760, ISSN: 0947-6539, DOI: 10.1002/chem.201805900
GASPARINI GIULIO, BANG EUN-KYOUNG, MOLINARD GUILLAUME, TULUMELLO DAVID V., WARD SANDRA, KELLEY SHANA O., ROUX AURELIEN, SAKAI NAOM: "Cellular Uptake of Substrate-Initiated Cell-Penetrating Poly(disulfide)s", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 136, no. 16, 23 April 2014 (2014-04-23), pages 6069 - 6074, XP055860766, ISSN: 0002-7863, DOI: 10.1021/ja501581b
See also references of EP 4140500A4
Attorney, Agent or Firm:
UNITALEN ATTORNEYS AT LAW (CN)
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