Title:
APPLICATION OF METALLO-SUPRAMOLECULAR BRANCHED POLYMERS IN CRYO-ELECTRON MICROSCOPY SAMPLE PREPARATION
Document Type and Number:
WIPO Patent Application WO/2022/257966
Kind Code:
A1
Abstract:
Disclosed are methods and compositions for use in preparing dynamic biological macromolecules for high-resolution cryo-electron microscopy or cryo-electron tomography imaging. The compositions contain a metallo-supramolecular branched polymer with a positive zeta potential, containing a hydrophilic polymer segment, a chelating chemical group, and a metal ion. The hydrophilic polymer segment is covalently bonded to the chelating chemical group that is in turn bonded to the metal ion via a dative bond. The methods and compositions can be used to improve particle distribution in vitreous ice and/or to change particle orientations in vitreous ice. Accordingly, the methods and compositions can be utilized to improve particle distribution for high resolution structure determination using single-particle cryo-EM.
Inventors:
DANG SHANGYU (CN)
XU YIXIN (CN)
WANG YUFENG (CN)
WANG LANG (CN)
XU YIXIN (CN)
WANG YUFENG (CN)
WANG LANG (CN)
Application Number:
PCT/CN2022/097629
Publication Date:
December 15, 2022
Filing Date:
June 08, 2022
Export Citation:
Assignee:
UNIV HONG KONG (CN)
DANG SHANGYU (CN)
XU YIXIN (CN)
DANG SHANGYU (CN)
XU YIXIN (CN)
International Classes:
G01N1/42; G01N23/04; G01N23/2202
Domestic Patent References:
| WO2018100562A1 | 2018-06-07 |
Foreign References:
| EP3790036A2 | 2021-03-10 | |||
| CN105940291A | 2016-09-14 | |||
| CN110945340A | 2020-03-31 | |||
| CN105044133A | 2015-11-11 |
Attorney, Agent or Firm:
LIU, SHEN & ASSOCIATES (CN)
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