Title:
AUTOMATED IDENTIFICATION OF PEPTIDES
Document Type and Number:
WIPO Patent Application WO2002021139
Kind Code:
A3
Abstract:
A fully automated, computer-mediated and user-independent method is described to identify and characterize a peptide sequence present in a peptide database that corresponds to an experimental peptide. The method identifies the corresponding sequence if it is present in the database, without the need for a skilled observer to choose from amongst a list of possible matches. By using an automated back-read process, the present method can uniquely identify a corresponding peptide sequence in a database based on a single matching peptide sequence. The method also permits mapping of mass spectral data to sequences in peptide or nucleotide databases for unambiguous identification of exons; determining a correct reading frame; identifying artefacts and errors in sequences; identifying mutations and polymorphisms; identifying post-translational modifications; and identifying exon-intron boundaries. Also provided are a computer-readable medium comprising instructions for causing a computer to perform the disclosed methods; a computer comprising such instructions; and a peptide or nucleic acid database, a computer-readable file or list, or a display comprising information obtained by performing the disclosed methods.
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Inventors:
TOWNSEND ROBERT REID (GB)
ROBINSON ANDREW WILLIAM (GB)
ROBINSON ANDREW WILLIAM (GB)
Application Number:
PCT/GB2001/004034
Publication Date:
February 06, 2003
Filing Date:
September 10, 2001
Export Citation:
Assignee:
OXFORD GLYCOSCIENCES UK LTD (GB)
TOWNSEND ROBERT REID (GB)
ROBINSON ANDREW WILLIAM (GB)
TOWNSEND ROBERT REID (GB)
ROBINSON ANDREW WILLIAM (GB)
International Classes:
G01N33/68; G06K9/00; G16B30/10; (IPC1-7): H01J49/04
Domestic Patent References:
| WO1999062930A2 | 1999-12-09 |
Other References:
DONGRE A R ET AL: "Emerging tandem-mass-spectrometry techniques for the rapid identification of proteins", TRENDS IN BIOTECHNOLOGY, ELSEVIER PUBLICATIONS, CAMBRIDGE, GB, vol. 15, no. 10, 1 October 1997 (1997-10-01), pages 418 - 425, XP004090548, ISSN: 0167-7799
LINDH I ET AL: "De novo sequencing of proteolytic peptides by a combination of C-terminal derivatization and nano-electrospray/collision-induced dissociation mass spectrometry", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, ELSEVIER SCIENCE INC., NEW YORK, NY, US, vol. 11, no. 8, August 2000 (2000-08-01), pages 673 - 686, XP004210133, ISSN: 1044-0305
BARTELS C: "FAST ALGORITHM FOR PEPTIDE SEQUENCING BY MASS SPECTROSCOPY", BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY, WILEY, LONDON, GB, vol. 19, 1990, pages 363 - 368, XP001051563, ISSN: 0887-6134
LINDH I ET AL: "De novo sequencing of proteolytic peptides by a combination of C-terminal derivatization and nano-electrospray/collision-induced dissociation mass spectrometry", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, ELSEVIER SCIENCE INC., NEW YORK, NY, US, vol. 11, no. 8, August 2000 (2000-08-01), pages 673 - 686, XP004210133, ISSN: 1044-0305
BARTELS C: "FAST ALGORITHM FOR PEPTIDE SEQUENCING BY MASS SPECTROSCOPY", BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY, WILEY, LONDON, GB, vol. 19, 1990, pages 363 - 368, XP001051563, ISSN: 0887-6134
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