BACTERIUM FOR ENGINEERING ACARBOSE, PREPARATION METHOD THEREFOR, AND APPLICATION THEREOF

Title:

BACTERIUM FOR ENGINEERING ACARBOSE, PREPARATION METHOD THEREFOR, AND APPLICATION THEREOF

Document Type and Number:

WIPO Patent Application WO/2018/171400

Kind Code:

A1

Abstract:

Provided are a bacterium for engineering acarbose, a preparation method therefor, and an application thereof. The bacterium for engineering acarbose is an acarbose producing Actinoplanes bacterium or a derivative strain thereof, in which one or both of the following genes are inactivated: (i) a gene M that codes a polypeptide having the sequence shown in SEQ ID NO: 2 or having a sequence identity of at least 80%, 90%, 95%, or 99% with the sequence shown in SEQ ID NO:2; and (ii) a gene N that codes a polypeptide having the sequence shown in SEQ ID NO: 3 or having a sequence identity of at least 80%, 90%, 95%, or 99% with the sequence shown in SEQ ID NO:3. Production of acarbose using the bacterium for engineering acarbose can reduce impurities of a component A and/or a component B in a product.

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Inventors:

HUANG JUN (CN)
LI MEIHONG (CN)
ZHOU JUN (CN)
LIN JIATAN (CN)
YU ZHEN (CN)

Application Number:

PCT/CN2018/077732

Publication Date:

September 27, 2018

Filing Date:

March 01, 2018

Export Citation:

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Assignee:

ZHEJIANG HISUN PHARM CO LTD (CN)

International Classes:

C12N1/21; C12P19/26; C12R1/045

Domestic Patent References:

WO2012016960A1

2012-02-09

Foreign References:

CN103305544A

2013-09-18

Other References:

YU, ZHEN ET AL.: "Effect of treY Gene Inactivation on reducing of acarbose component C in Actinoplanes 8-22", MICROBIOLOGY CHINA, vol. 42, no. 7, 20 July 2015 (2015-07-20), pages 1301 - 1306, ISSN: 0253-2654
WANG ET AL: "Reconstruction and in silico analysis of an Actinoplanes sp SE 50/110 genome-scale metabolic model for acarbose production", FRONTIERS IN MICROBIOLOGY, vol. 6, 632, 25 June 2015 (2015-06-25), XP055610647, ISSN: 1664-302X

Attorney, Agent or Firm:

BOSS & YOUNG PATENT AND TRADEMARK LAW OFFICE (CN)

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